pSC101 (ATCC® 37032)

Applications: vector with low copy number  /  Depositors: SN Cohen

Permits and Restrictions

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Designations pSC101
Depositors SN Cohen
Biosafety Level 1
Vector Information
Size (kb): 9.263
Vector: pSC101 (plasmid)
Construction: natural isolate
Marker(s):tetR
Construct size (kb): 9.263
Features: marker(s): tetR
replicon: pSC101
Applications
vector with low copy number
Comments
General purpose plasmid vector.
Media Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110

Cohen SN, Change AC. Revised interpretation of the origin of the pSC101 plasmid. J. Bacteriol. 132: 734-737, 1977. PubMed: 334752

Bernardi A, Bernardi F. Complete sequence of pSC101. Nucleic Acids Res. 12: 9415-9426, 1984. PubMed: 6096829

Shipped frozen