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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Other IDs
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Nucleotide (GenBank) :
VB0109
Cloning vector pACYC177.
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Vector Information
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Size (kb): 3.9400000572204590 Vector: pACYC177 (plasmid) Construction: p15A, Tn903, Tn3 Marker(s):ampR,kanR Construct size (kb): 3.940000057220459 Features: marker(s): ampR, kanR
replicon: p15A
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Comments
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Low copy number plasmid vector containing the replication system of miniplasmid p15A. When HincII is nucleotide 1, Tn3 region extends from 3026-756 and beta-lactamase from 369-615. The promoter and part of the coding region for the Tn3 repressor (3025-3515) have been deleted. The Tn903 (1595-3025) region includes kanR (1922-2734).
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References
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Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110
Rose RE. The nucleotide sequence of pACYC177. Nucleic Acids Res. 16: 356, 1988. PubMed: 3340534
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Shipping Information
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Shipped: Frozen glycerol stock of E. col containing the plasmid.
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