pACYC177 (ATCC® 37031)

Other IDs:

Nucleotide (GenBank) : VB0109 Cloning vector pACYC177.

 /  Depositors: SN Cohen

Permits and Restrictions

View Permits

Designations pACYC177
Depositors SN Cohen
Other IDs

Nucleotide (GenBank) : VB0109 Cloning vector pACYC177.

Biosafety Level 1
Vector Information
Size (kb): 3.9400000572204590
Vector: pACYC177 (plasmid)
Construction: p15A, Tn903, Tn3
Marker(s):ampR,kanR
Construct size (kb): 3.940000057220459
Features: marker(s): ampR, kanR
replicon: p15A
Comments
Low copy number plasmid vector containing the replication system of miniplasmid p15A.
When HincII is nucleotide 1, Tn3 region extends from 3026-756 and beta-lactamase from 369-615. The promoter and part of the coding region for the Tn3 repressor (3025-3515) have been deleted. The Tn903 (1595-3025) region includes kanR (1922-2734).
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110

Rose RE. The nucleotide sequence of pACYC177. Nucleic Acids Res. 16: 356, 1988. PubMed: 3340534

Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of  E. col containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37031