pHC79 (ATCC® 37030)

Applications: vector for constructing cDNA libraries  /  Depositors: J Collins

Permits and Restrictions

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Designations pHC79
Depositors J Collins
Biosafety Level 1
Vector Information
Size (kb): 6.43
Vector: pHC79 (cosmid)
Construction: pBR322, Charon 4A
Marker(s):ampR,tetR
Construct size (kb): 6.43
Features: marker(s): ampR, tetR
replicon: pMB1
terminator: none
Applications
vector for constructing cDNA libraries
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
General purpose cosmid vector derived from pBR322.
pBR322 was partially digested with Sau3A and ligated with an 0.65 BglII lambda fragment, generating a BglII site (pBR322 positions 1459-1666 deleted). A 1.65 kb BglII fragment from Charon 4A was ligated into the BglII site.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Hohn B, Collins J. A small cosmid for efficient cloning of large DNA fragments. Gene 11: 291-298, 1980. PubMed: 6260575

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried stock of Escherichia coli containing the plasmid.

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