Crithidia luciliae (Strickland) Wallace and Clark (ATCC® 30258)

Strain Designations: [ATCC 14765]  /  Depositor: FG Wallace, T Clark  /  Biosafety Level: 1

Permits and Restrictions

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Strain Designations [ATCC 14765]
Detection of lupus erythematosus
Assay of unconjugated pteridines; listed here as ATCC 14765
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Green bottle fly, Phaenicia sericata, Minneapolis, MN, 1958
Product Format frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:

Live Cultures:
See Protocols section for handling information
Type Strain yes
Flagellate parasites from a fly
Effects of purine starvation
Riboprinting and taxonomy
Thermal regulation of oxygen-scavenging enzymes
Multiple distinct site-specific elements in miniexon arrays
Cyclopropane fatty acid
Medium ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 355: Crithidia medium
ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
Growth Conditions
Temperature: 25°C
Culture System: Axenic
Cryopreservation Harvest and Preservation
  1. Prepare a 10% (v/v) sterile DMSO solution in fresh ATCC Medium 355. 
  2. Transfer a culture at peak density to centrifuge tubes and centrifuge at 525 x g for 5 minutes.
  3. Remove the supernatant and resuspend the cells in ATCC medium 355 to a concentration of 2 x 106 to 2 x 107 cells/mL.
  4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be between 106 and 107 cells/mL and 5% (v/v) DMSO.
  5. Distribute the cell suspension in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).  The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min.
  6. Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  
  7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2 to 3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial.
  9. Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 mL of fresh ATCC medium 355 in a 16 x 125 mm screw-capped test tube. Incubate upright at 25°C with caps screwed on tightly.
Mycoplasma Unknown
Name of Depositor FG Wallace, T Clark
Special Collection NSF - Protistology
Year of Origin 1958

Aarden LA, et al. Immunology of DNA. III. Crithidia luciliae, a simple substrate for the determination of anti-dsDNA with the immunofluorescence technique. Ann. N.Y. Acad. Sci. 254: 505-515, 1975. PubMed: 52321

Alleman MM, et al. Crithidia luciliae: Effect of purine starvation on S-adenosyl-L-methionine uptake and protein methylation. Exp. Parasitol. 81: 519-528, 1995. PubMed: 8542993

Analytical microbiology. vol. 2New York: Academic Press; 1972.

Branquinha MH, et al. Ubiquity of cysteine-and metalloproteinase activites in a wide range of trypanosomatids. J. Eukaryot. Microbiol. 43: 131-135, 1996. PubMed: 8720943

Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198

Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441

Daggett PM, Decker JE. Some physiological alterations accompanying infectivity to mammals by four genera of Trypanosomatidae. Comp. Biochem. Physiol. 59: 363-366, 1978.

Emtage MA, Bremner TA. Thermal regualtion of active oxygen-scavenging enzymes in Crithidia Luciliae thermophila. J. Parasitol. 79: 809-814, 1993.

Faria e Silva PM, et al. Herpetomonas roitmani (Fiorini et al., 1989) n. comb.: a trypanosomatid with a bacterium-like endosymbiont in the cytoplasm. J. Protozool. 38: 489-494, 1991. PubMed: 1920148

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Freymuller E, Camargo EP. Ultrastructural differences between species of trypanosomatids with and without endosymbionts. J. Protozool. 28: 175-182, 1981. PubMed: 7024533

Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159

Goncanlves De Lima VM, et al. Comparison of six isoenzymes from 10 species of Crithidia. J. Protozool. 29: 397-401, 1982.

Wallace FG, Clark TB. Flagellate parasites of the fly, Phaenicia sericata (Meigen). J. Protozool. 6: 50-61, 1959.

Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238

Sontheimer RD, Gilliam JN. An immunofluorescence assay for double-stranded DNA antibodies using the Crithidia luciliae kinetoplast as a double-stranded DNA substrate. J. Lab. Clin. Med. 91: 550-558, 1978. PubMed: 347011

Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation