Trichomonas vaginalis Donne (ATCC® 30236)

Organism: Trichomonas vaginalis Donne  /  Depositor: BM Honigberg

Permits and Restrictions

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Strain Designations JH 31A #4
Application
Sexually Transmitted Disease Research
Biosafety Level 2
Isolation
endocervical swab, Johns Hopkins Hosp., Baltimore, MD, 1963
Product Format frozen
Type Strain no
Comments
Very sensitive to metronidazole.
beta-tubulin genes
presence of HSP70 and HSP60 homologs
Virus RNAs
Inhibition of growth by difluoromethyl-ornithine
Heterogeneity of trichomonal isolates
geographic variation
Medium Medium 2154: LYI Entamoeba medium
Growth Conditions
Temperature: 35.0°C
Duration: axenic; anaerobic
Protocol: at pH 6.0
Subcultivation
Protocol: at pH 6.0
Cryopreservation

1.  Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. The cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.

2.  Adjust the concentration of cells to 2 x 106 - 2 x 107/ml in fresh medium.

3.  While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium.

a) Add 1.0 ml of DMSO to an ice cold 20 x 150 mm screw-capped test tube;

b) Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 ml of ice cold medium;

c) Invert several times to dissolve the DMSO;

d) Allow to warm to room temperature.

4.  Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/ml and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.

5.  Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6.   Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at        -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately

      -1°C/min.)  

7.  The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.

8.   To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.

9.   Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 ml of ATCC medium 361 (completed with serum) or 13 ml ATCC Medium 2154 adjusted to pH 6.0.

10.      Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 361 or on a 15° horizontal slant for medium 2154).

Name of Depositor BM Honigberg
Year of Origin 1963
References

Katiyar SK, Edlind TD. B-tubulin genes of Trichomonas vaginalis. Mol. Biochem. Parasitol. 64: 33-42, 1994. PubMed: 8078521

Tai JH, et al. The divergence of Trichomonas vaginalis virus RNAs among various isolates of Trichomonas vaginalis. Exp. Parasitol. 76: 278-286, 1993. PubMed: 8500587

Gillin FD, et al. Inhibition of growth of Giardia lamblia by difluoromethylornithine, a specific inhibitor of polyamine biosynthesis. J. Protozool. 31: 161-163, 1984. PubMed: 6330350

Alderete JF, et al. Heterogeneity of Trichomonas vaginalis and discrimination among trichomonal isolates and subpopulations with sera of patients and experimentally infected mice. Infect. Immun. 49: 463-468, 1985. PubMed: 2411656

Krieger JN, et al. Geographic variation among isolates of Trichomonas vaginalis: demonstration of antigenic heterogeneity by using monoclonal antibodies and the indirect immunofluorescence technique. J. Infect. Dis. 152: 979-984, 1985. PubMed: 2413147

Bozner P. Immunological detection and subcellular localization of HSP70 and HSP60 homologs in Trichomonas vaginalis. J. Parasitol. 83: 224-229, 1997. PubMed: 9105301

Vanacova S, et al. Characterization of Trichomonad species and strains by PCR fingerprinting. J. Eukaryot. Microbiol. 44: 545-552, 1997. PubMed: 9435127

Mattos A, et al. Fine structure and isozymic characterization of trichomonadid protozoa. Parasitol. Res. 83: 290-295, 1997. PubMed: 9089728

Rosset I, et al. Scanning electron microscopy in the investigation of the in vitro hemolytic activity of Trichomonas vaginalis. Parasitol. Res. 88: 356-359, 2002. PubMed: 11999024

Davis-Hayman SR, et al. Trichomonas vaginalis: analysis of a heat-inducible member of the cytosolic heat-shock-protein 70 multigene family. Parasitol. Res. 86: 608-612, 2000. PubMed: 10935914

Benchimol M. Hydrogenosome morphological variation induced by fibronectin and other drugs in Trichomonas vaginalis and Tritrichomonas foetus. Parasitol. Res. 87: 215-222, 2001. PubMed: 11293569

Lecke SB, et al. Trichomonas vaginalis: microtubule cytoskeleton distribution using fluorescent taxoid. Exp. Parasitol. 102: 113-116, 2002.

Cross References

Nucleotide (GenBank) : L29561 Trichomonas vaginalis (clones TVARD1278 and TV1RD1267) small subunit ribosomal RNA gene, 3' end; 5.8S ribosomal RNA gene, complete sequence; large subunit ribosomal RNA gene, 5'end.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation