Mycoplasma synoviae Olson et al. emend. Jordan et al.

1. Follow instructions as suggested for the culturing of Mollicutes:

                PROCEDURES FOR PROPAGATING MOLLICUTES:

1. Allow the vial to thaw on bench top.
2. Using a Pasteur or 1.0 mL pipette, withdraw entire contents of vial.
3. Aseptically transfer this aliquot back into the maintenance media tube. Mix well.
4. Perform a serial dilution by making 1:10 dilutions in broth. Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth. Many strains will die out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze drying process often inhibits growth.
5. Use an uninoculated tube of broth to serve as a control.
6. Plates may be inoculated to check colonial morphology. You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony­-forming units. However, not all strains do well on solid medium.
7. Incubate all tubes and plates under the recommended conditions and appropriate temperature. The time necessary for growth will vary from strain to strain. Growth on plates generally requires additional incubation. 
8. Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both. 

2. This strain starts to show a color change in the first few dilution tubes within 72+ hours. Additional incubation of 5-­7 days may be required for growth on agar.
3. Subsequent fresh transfers will grow more rapidly than the original rehydrated culture.  This strain produces light turbidity.

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