Escherichia coli (Migula) Castellani and Chalmers (ATCC® 11303)

Strain Designations: B [CIP 103914, NCIB 11595, NRC 745]  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations B [CIP 103914, NCIB 11595, NRC 745]
Application
Bacteriophage host
Produces aspartate transaminase aspartate aminotransferase
Testing
Testing of aerosol containment on cell sorters
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain no
Comments
Readily produces colonial mutants.
T cell rosette test
Medium Medium 129: Nutrient agar/stabs/broth with 0.5% NaCl
Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor SE Luria
References

Orye E, et al. A new, simple and rapid method for enumerating human T lymphocytes in full blood: the E. coli (ATCC 11303) Rosette test. J. Immunol. Methods 60: 369-377, 1983. PubMed: 6189915

Wood LL, Carlton GJ. Process for preparing phenylalanine. US Patent 4,710,467 dated Dec 1 1987

Schmid I, et al. Biosafety guidelines for sorting of unfixed cells. Cytometry 28: 99-117, 1997. PubMed: 9181299

Bacteriophage-based detection methods. Washington, DC:American Public Health Association;APHA APHA2001-10.72, 2001

8310 B: Growth inhibition test with freshwater ciliate Colpidium campylum. Washington, DC:American Public Health Association;Standard Methods for the Examination of Water and Wastewater, 2005 APHA8310 - Ciliated Protozoa;.