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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Designations
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pEX_YFP6-SV40LTa_NLS plasmid in E. coli
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Depositors
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Alliance for Cell Signaling
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Applications
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Expresses SV40 Large T antigen_NLS triplet, N-terminally tagged with 6x YFP, under the control of the CMV promoter in WEHI-231 cells ( ATCC CRL-1702). |
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Vector
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Construct size (kb): 8.541 Type: expression Marker for bacterial selection: KanR Marker for mammalian selection: Neomycin Promotor: CMV Putative target: Nucleus
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Insert
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DNA:cDNA Insert size (kb): 0.081 SV40 Large T antigen_NLS triplet
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Putative ID
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SV40 Large T antigen_NLS triplet
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Comments
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Expresses SV40 Large T antigen_NLS triplet, N-terminally tagged with 6x YFP, under the control of the CMV promoter in WEHI-231 cells ( ATCC CRL-1702). See the Alliance for Cellular Signaling (AfCS) website for more information (www.sinaling-gateway.org) More specific information on this construct can be found by searching the following website using the ATCC number:
www.signaling-gateway.org/data/plasmid/Plasmid.cgi See http://afcs.lbl.gov/reports/v1/DA0002/DA0002.pdf for details on the subcellular localization marker clones. Restriction digests of the clone give the following size bands (in kb): NcoI - 5683, 1904, 703, 251
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