pEX_CFP6-SV40LTa_NLS plasmid in E. coli (ATCC® 10089303)

Organism: SV40 virus  /  Depositors: Alliance for Cellular Signaling

Permits and Restrictions

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Designations pEX_CFP6-SV40LTa_NLS plasmid in E. coli
Species SV40 virus
Depositors Alliance for Cellular Signaling
Applications
Expresses SV40 Large T antigen_NLS triplet, N-terminally tagged with 6x CFP, under the control of the CMV promoter in WEHI-231 cells (ATCC CRL-1702).
Vector
Construct size (kb): 8.541
Type: expression
Marker for bacterial selection: KanR
Marker for mammalian selection: Neomycin
Promotor: CMV
Putative target: Nucleus
Insert
DNA:cDNA
Insert size (kb): 0.081
SV40 Large T antigen_NLS triplet
Putative ID
SV40 Large T antigen_NLS triplet
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Related Products
Component of: MBA-91
Biosafety Level 1
Shipping Information Escherichia coli GC10
Comments
Expresses SV40 Large T antigen_NLS triplet, N-terminally tagged with 6x CFP, under the control of the CMV promoter in WEHI-231 cells (ATCC CRL-1702).
See the Alliance for Cellular Signaling (AfCS) website for more information (www.sinaling-gateway.org)
More specific information on this construct can be found by searching the following website using the ATCC number: www.signaling-gateway.org/data/plasmid/Plasmid.cgi
See http://afcs.lbl.gov/reports/v1/DA0002/DA0002.pdf for details on the subcellular localization marker clones.
Restriction digests of the clone give the following size bands (in kb): NcoI - 5683, 1904, 703, 251
Restrictions This product's use is governed by the Limited Use License.. For information on purchasing a license to use this product for research (for-profit entities) or commercial purposes (any entity) other than those permitted in the limited use label license, contact the Licensing Department, Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, California 92008. Phone (760) 603-7200 or outlicensing@lifetech.com
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