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  • DMEM: F-12 Medium (ATCC® 30-2006)

    ATCC® Number: 30-2006
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    DMEM: F-12 Medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • RPMI-1640 Medium (ATCC® 30-2001)

    ATCC® Number: 30-2001
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate, for use in incubators using 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

    For-Profit:   $20.00 Non-Profit:   $20.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Water, Cell Culture Grade (ATCC® 30-2205)

    ATCC® Number: 30-2205
    Quantity: 500 mL
    Storage: Store cell culture grade water at room temperature (15°C to 30°C). Shelf life of 36 months when properly stored.
    Applications:

    For use as a diluent or for reconstituting powdered media and salt solutions.

    For-Profit:   $11.00 Non-Profit:   $11.00

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    Product Format: 500 mL
    Comments:
    Cell culture tested.
  • Phosphate Buffered Saline Without Calcium Or Magnesium (ATCC® SCRR-2201)

    ATCC® Number: SCRR-2201
    Quantity: 500 mL
    Storage: Store at ambient temperatures, between 15°C and 30°C.
    Applications:

    For washing cells (including embryonic stem cells), for use as a diluent, or for use as an inorganic base for standard media preparation.

    For-Profit:   $13.00 Non-Profit:   $13.00

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  • LYI Entamoeba Medium (ATCC® PRA-2154)

    ATCC® Number: PRA-2154
    Storage: 2 to 8°C or colder
    Applications: This medium is the recommended formulation for the axenic cultivation of Entamoeba, Hypotrichomonas, Mastigamoeba, Pentatrichomonas, Tetratrichomonas, Trichomitus, Trichomonas, and Tritrichomonas. It is provided freeze-dried, complete with 10% (1.5 mL) bovine serum, 1 vial prepares 15 mL of medium.

    For-Profit:   $65.00 Non-Profit:   $65.00

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    Product Format: freeze-dried
    Comments:
    Ready to use upon rehydration with sterile glass-distilled water.

    Media may not support growth of some strains; efficacy for growth of a particular strain should be determined empirically prior to regular work.
  • LB Broth (ATCC® 60-2100)

    ATCC® Number: 60-2100
    Quantity: 1 liter
    Storage: 15°C to 30°C (ambient temperature)
    Applications:

    Sterile liquid is ready to use.
    Luria-Bertani medium for propagation of bacteria for molecular biology applications.

    For-Profit:   $30.00 Non-Profit:   $30.00

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  • Eagle's Minimum Essential Medium (EMEM) (ATCC® 30-2003)

    ATCC® Number: 30-2003
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: Modified to contain Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. 

    For-Profit:   $19.00 Non-Profit:   $19.00

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    Product Format: 500 mL
    Comments: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Trypsin-EDTA Solution, 1X (ATCC® 30-2101)

    ATCC® Number: 30-2101
    Quantity: 100 mL
    Storage: Store Trypsin-EDTA solution frozen, between -5°C and -20°C. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
    Applications:

    0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. For cell line-specific information, please go to the appropriate product page on the web, refer to the product sheet supplied with the cell line, or contact ATCC Technical Service.

    For-Profit:   $13.00 Non-Profit:   $13.00

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    Product Format: frozen 100 mL
    Comments: NOTE:  Each cell line responds to ATCC® Trypsin-EDTA solution in a unique manner.  For optimum results, observe the cells during the dissociation process to avoid over-trypsinization.  For more information, please refer to the General Protocol for Using ATCC® Trypsin-EDTA Solution.  For cell line-specific information, please refer to the product sheet supplied with the cell line, or contact ATCC® Technical Service. ATCC® Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. This product contains phenol red.
  • AlphaMEM with Nucleosides without L-Glutamine. (ATCC® 30-2604)

    ATCC® Number: 30-2604
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    AlphaMEM (Alpha Minimum Essential Medium) is an enriched modification of MEM that is widely used in mammalian cell culture. This formulation is made in Earle’s salts and contains ribonucleosides and deoxyribonucleosides.

    For-Profit:   $31.00 Non-Profit:   $31.00

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    Product Format: 500 mL
    Comments: Supplement with 2 mM L-glutamine prior to use.
  • Trace Mineral Supplement (ATCC® MD-TMS)

    ATCC® Number: MD-TMS
    Quantity: Package of 5 (10 mL/vial)
    Storage: Store at 4 to 8°C
    Applications:

    ATCC® Trace Mineral Supplement is used as an additive in many ATCC® recommended media, especially for cultivating extremophiles. It speeds media preparation and reduces possible lab errors by combining several trace mineral ingredients into one sterile solution. Product is provided in an individual unit of use (10 mL).

    For-Profit:   $65.00 Non-Profit:   $65.00

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    Product Format: Package of 5 (10 mL/vial)
    Comments: Based on Wolfe's formulation