pUN70 [Y302, pNN336] (ATCC® 77268)

Applications: YC-type (centromeric) shuttle vectormutation detectionshuttle vectorvector permitting production of single-stranded DNAvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pUN70 [Y302, pNN336]
Depositors SJ Elledge
Biosafety Level 1
Vector Information
Size (kb): 5.6999998092651370
Vector: pUN70 (phagemid)
Promoters: Promoter lac
Construction: pUC18
Marker(s):URA3,ampR
Construct size (kb): 5.699999809265137
Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: M13+
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
mutation detection
shuttle vector
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; KpnI--5.2; BamHI--5.2; PvuII--5.0, 0.3.
YC-type shuttle vector permitting visual detection of recombinants by lacZ alpha complementation and production of ssDNA (in the same sense as lacZ mRNA).
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604

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