Canine herpesvirus 1 (ATCC® VR-1785)

Classification: Herpesviridae, Varicellovirus  /  Product Format: frozen

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Classification Herpesviridae, Varicellovirus
Agent Canine herpesvirus 1
Strain F 205 (Carmichael)
Common Name Canine herpesvirus
Biosafety Level 2
Product Format frozen
Storage Conditions -70°C
Comments
Derived by adaptation of primary cell culture product ATCC VR-647 to MDCK cells (ATCC CCL-34). ATCC VR-647 and ATCC VR-1785 have not been compared for sequence or infectivity in primary cell culture and tissue culture. Chloroform/ether sensitive, not known to hemagglutinate, sensitive to pH below 6.5 and heat labile.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
MDCK (ATCC CCL-34)
Growth Conditions
Temperature: 37°
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor JH Gillespie, New York State Veterinary College, Cornell University
Source
Kidney from 2-3 weeks old puppy
References

Carmichael LE, et al. Clinical and pathologic features of a fatal viral disease of newborn pups. Am. J. Vet. Res. 26: 803-814, 1965. PubMed: 5892835

Carmichael LE, et al. Identification of a cytopathogenic agent infectious for puppies as a Canine herpesvirus. Proc. Soc. Exp. Biol. Med. 120: 644-650, 1965. PubMed: 4221827

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation