NCI-H676B [H676B] (ATCC® HTB-179)

Organism: Homo sapiens, human  /  Disease: adenocarcinoma

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties suspension, multicell aggregates (gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures)
Biosafety Level 1
Disease adenocarcinoma
Age 63 years
Gender male
Ethnicity Caucasian
Applications
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment.
The cells produce a abnormal size p53 mRNA (2.3 kb) as well as the normal size mRNA (2.8 kb).
The line does not exhibit any gross structural DNA abnormalities.
The cells produce mucin, and occasionally signet ring cells are formed.
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hypodiploid cell line; modal number = 43; range = 39 to 47. Marker chromosomes consisted of over 70% of the chromosomes in each cell complement. Among the identifiable markers were t(1q8q), der(3)t(3;?)(q29;?), t(2q7q), der(9)t(9;14)(p13;q11) and der(11)t(?q23;?). All markers had a single copy per cells. The N5, N9, N10, N17, N18, N20, N21 and the X were the only structurally normal chromosomes found. Except for the paired N5, all others had single copies per cell.
Derivation
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment.
Clinical Data
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment.
male
Caucasian
63 years
Genes Expressed
mucin
Cellular Products
mucin
Comments
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment.
The cells produce a abnormal size p53 mRNA (2.3 kb) as well as the normal size mRNA (2.8 kb).
The line does not exhibit any gross structural DNA abnormalities.
The cells produce mucin, and occasionally signet ring cells are formed.
Complete Growth Medium ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  • 0.02 mg/ml insulin
  • 0.01 mg/ml transferrin
  • 25 nM sodium selenite (final conc.)
  • 50 nM Hydrocortisone (final conc.)
  • 1 ng/ml Epidermal Growth Factor (do not filter)
  • 0.01 mM ethanolamine (final conc.)
  • 0.01 mM phosphorylethanolamine (final conc.)
  • 100 pM triiodothyronine (final conc.)
  • 0.5% (w/v) bovine serum albumin (final conc.)
  • 10 mM HEPES
  • 0.5 mM sodium pyruvate (final conc.)
  • extra 2mM L-glutamine (for final conc. of 4.5mM)

Subculturing
Protocol: This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 82.5%; fetal bovine serum, 10%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 9
D13S317: 10
D16S539: 11,12
D5S818: 11
D7S820: 12
THO1: 9.3
TPOX: 8,11
vWA: 16,19
Isoenzymes
AK-1, 2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 0
PGM1, 1-2
PGM3, 2
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1984
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures

gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures

gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures