GK-5 (ATCC® CRL-1834)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed  / 

Permits and Restrictions

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Organism Homo sapiens, human
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.
The cells secrete very low levels (less than 10 ng/ml) of human kappa light chain.
The line has been used successfully to prepare human hybridomas.
Derivation
GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.
Genes Expressed
immunoglobulin; monoclonal antibody; against human kappa light chain
Cellular Products
immunoglobulin; monoclonal antibody; against human kappa light chain
Comments
GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.
The cells secrete very low levels (less than 10 ng/ml) of human kappa light chain.
The line has been used successfully to prepare human hybridomas.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • fetal bovine serum to a final concentration of 10%

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor JF Kearney
References

Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984

Satoh J, et al. Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984

Satoh J, et al. Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036