HEKPlus Boost Solution (ATCC® ACS-4003)

Product Format 50 mL
Storage Conditions Store at 2°C to 8°C. Do not freeze. Protect from light.
Comments The addition of peptone following transfection has been shown to increase extracellular protein expression yields in transient transfection protocols.

HEKPlus Boost Solution should be added post transfection to a final concentration of 10% in complete HEKPlus SFM. The solution contains 0.1% Poloxamer 188, a surfactant shown to enhance cell yield and reduce cell aggregation. HEKPlus Boost Solution also contains human transferrin and recombinant human insulin.

This solution has been optimized for use with ATCC® HEK293T/17 SF suspension cells (ATCC ACS-4500), HEKPlus SFM medium (ATCC ACS-4002) and GeneXPlus transfection reagent (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

Intended Use This product is intended for research use only. It is not intended for any animal or human therapeutic or diagnostic use.
Biosafety Level 1
Introduction HEKPlus Boost Solution is a 5% solution of enzymatically hydrolyzed peptone in HEKPlus SFM serum-free, xeno-free, and defined medium.
Quality Control Specifications

Each lot of solution is tested for osmolality, pH, sterility, mycoplasma and endotoxin.

Test

 

Specification

 

pH

 

7.0 to 7.6

 

Osmolality

 

500 to 534 mOsm/kg H2O

 

Sterility Testing

 

Pass

 

Mycoplasma

 

Pass

 

pH 7.0 to 7.6
Osmolality 500 to 534 mOsm/kg H2O
Endotoxin Pass
Sterility Testing pass
Functional Testing Sterility Testing: Pass
Mycoplasma (testing) pass
Basic Documentation
FAQs
  1. HEKPlus Protein Expression System Parameters
    Cell density at transfection, ratio of DNA to GeneX
    Date Updated: 3/27/2014
  2. GeneXPlus transfection reagent
    GeneX
    Date Updated: 3/27/2014
  3. HEKPlus Protein Expression System vectors
    Expression vectors containing the SV40 origin of replication should be used. These vectors permit more plasmid copies to persist in the transiently transfected cells.
    Date Updated: 3/27/2014
  4. HEKPlus SFM supplements
    Complete culture medium is prepared by adding L-glutamine to the HEK
    Date Updated: 3/27/2014
  5. HEKPlus Protein Expression System time for optimal protein expression
    It usually takes 2 to 3 days to see optimal expression of intracellular proteins and 6 days for optimal expression of secreted proteins. It is recommended that a time course be performed for...
    Date Updated: 3/27/2014
  6. Advantage of using HEKPlus Protein Expression System
    The HEK
    Date Updated: 3/27/2014
  7. HEKPlus Protein Expression System advantages
    The HEK
    Date Updated: 3/27/2014
  8. HEKPlus Protein Expression System to express hard-to-express proteins
    Yes. The HEK
    Date Updated: 3/27/2014
  9. HEKPlus Protein Expression System Scale
    The HEK
    Date Updated: 3/27/2014
  10. HEKPlus Protein Expression System secreted proteins
    It is recommended to collect cell supernatants at day 6 post-transfection. However, one may want to perform a time course study to determine optimal time for collection post-transfection for each...
    Date Updated: 3/27/2014
  11. HEK293T/17 SF cells are clumping
    No. Small aggregates are easily dispersed with minimal pipetting and do not affect the performance of the cell line.
    Date Updated: 3/27/2014
  12. Replenishing medium for HEK293T/17 SF cells
    Yes. Incomplete media changes affect the cell viability and recovery. Spent medium should be completely removed and replaced with fresh medium every 2 to 3 days.
    Date Updated: 3/27/2014
  13. Media volume for HEK293T/17 SF cells
    For protein expression, it is important to have adequate shaking in the culture vessel. We usually add culture medium to 20% volume of a shaker flask.
    Date Updated: 3/27/2014