Rickettsia parkeri (ATCC® VR-1637)

Classification: Rickettsiaceae, Rickettsieae  /  Product Format: frozen 1 mL per vial

Classification Rickettsiaceae, Rickettsieae
Agent Rickettsia parkeri
Strain Maculatum C
Biosafety Level 3
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host CPE, rounding
Recommended Host Vero (ATCC® CCL-81™)
Growth Conditions Temperature: 35°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of agent (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb by centrifugation at 800 x g for 30 minutes. End adsorption by adding virus growth medium.
Incubation: 7-14 days
Effect on Host CPE, rounding
Name of Depositor GA Dasch, Naval Medical Research Center