CATH.a (ATCC® CRL-11179)

Organism: Mus musculus, mouse  /  Cell Type: neuron  /  Tissue: brain  / 

Organism Mus musculus, mouse
Tissue brain
Cell Type neuron
Product Format frozen
Culture Properties mixed: adherent and suspension
Biosafety Level 2 [Cells contain SV-40 viral DNA sequences]
Storage Conditions liquid nitrogen vapor phase
Derivation
The CATH.a cell line was established from cultures of a tumor that arose in the brain of a transgenic mouse.
Genes Expressed
dopamine; norepinephrine; synaptophysin
Tumorigenic Yes
Effects
Yes, in syngeneic mice
Comments The transgene contain the SV40 T antigen coding sequence linked to a 783 bp portion of the rat tyrosine hydroxylase gene.
The cells express tyrosine hydroxylase and dopamine beta-hydroxylase, and contain neurofilaments.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 88%; horse serum, 8%; fetal bovine serum, 4%
Subculturing
Cultures can be maintained by transferring floating cells to additional flasks.Subculture attached cells using 0.12% trypsin without EDTA. Remove Trypsin by neutralization and centrifugation.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor Trustees of Tufts University
References

Chikaraishi DM. Expression of a target gene in transgenic mammals with 5' flanking sequences of the rat tyrosine hydroxylase gene. US Patent 5,591,626 dated Jan 7 1997

Basic Documentation
References

Chikaraishi DM. Expression of a target gene in transgenic mammals with 5' flanking sequences of the rat tyrosine hydroxylase gene. US Patent 5,591,626 dated Jan 7 1997