CATH.a (ATCC® CRL-11179)

Organism: Mus musculus, mouse  /  Cell Type: neuron  /  Tissue: brain  / 

Organism Mus musculus, mouse
Tissue brain
Cell Type neuron
Product Format frozen
Culture Properties mixed: adherent and suspension
Biosafety Level 2 [Cells contain SV-40 viral DNA sequences]

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions liquid nitrogen vapor phase
Derivation
The CATH.a cell line was established from cultures of a tumor that arose in the brain of a transgenic mouse.
Genes Expressed
dopamine; norepinephrine; synaptophysin
Tumorigenic Yes
Effects
Yes, in syngeneic mice
Comments The transgene contain the SV40 T antigen coding sequence linked to a 783 bp portion of the rat tyrosine hydroxylase gene.
The cells express tyrosine hydroxylase and dopamine beta-hydroxylase, and contain neurofilaments.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 88%; horse serum, 8%; fetal bovine serum, 4%
Subculturing
Cultures can be maintained by transferring floating cells to additional flasks.Subculture attached cells using 0.12% trypsin without EDTA. Remove Trypsin by neutralization and centrifugation.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor Trustees of Tufts University
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Chikaraishi DM. Expression of a target gene in transgenic mammals with 5' flanking sequences of the rat tyrosine hydroxylase gene. US Patent 5,591,626 dated Jan 7 1997

Basic Documentation
References

Chikaraishi DM. Expression of a target gene in transgenic mammals with 5' flanking sequences of the rat tyrosine hydroxylase gene. US Patent 5,591,626 dated Jan 7 1997