Influenza A virus (H1N1) (ATCC® VR-1469)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 1 mL per vial

Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H1N1)
Strain A/PR/8/34
Applications
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images ATCC VR-1469 micrograph images
Comments
VR-1469® is a TC-adapted item derived from virus passaged in eggs (ATCC® VR-95™).
Effect on Host
CPE, rounding, degeneration, sloughing
Recommended Host
MDCK cells (ATCC® CCL-34™)
Growth Conditions Temperature: 35°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, rounding, degeneration, sloughing
Name of Depositor ATCC®, M Coleman, Centers for Disease Control and Prevention
Special Collection NCRR Contract
Source Patient in Puerto Rico, 1934
References

Francis T. Immunological relationships of strains of filterable virus recovered from cases of human influenza. Proc. Soc. Exp. Biol. Med. 32: 1172-1175, 1935.