Hs 766T (ATCC® HTB-134)

Organism: Homo sapiens, human  /  Cell Type: Epithelial  /  Tissue: pancreas; derived from metastatic site: lymph node  /  Disease: pancreatic carcinoma

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Organism Homo sapiens, human
Tissue pancreas; derived from metastatic site: lymph node
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease pancreatic carcinoma
Age 46 years
Gender male
Ethnicity Caucasian
Applications

This cell line is a suitable transfection host.

Storage Conditions liquid nitrogen vapor temperature
Karyotype (P11) hyperdiploid with mode = 60; banded markers; lacking Y chromosomes
Derivation
The Hs 766T line was isolated by R. Owens et al. at the Naval Biosciences Laboratory in 1973 from a pancreatic carcinoma metastatic to a lymph node.
Derived from metastatic site, lymph node
Clinical Data
46 years
Caucasian
male
Tumorigenic Yes
Effects
Yes, in immunosuppressed mice
Comments
The epithelial nature of the cell strain selected was confirmed by electron microscopy as microvilli and desmosomes were observed.
Tests conducted at an early passage indicated that the cells exhibited many properties observed only in tumor lines. The karyotype was abnormal and the cells formed colonies in Methocel and on contact inhibited monolayers.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation

Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,12
D5S818: 11
D7S820: 10
THO1: 6,9.3
TPOX: 8
vWA: 18
Isoenzymes
AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1-2
PGM3, 1
Name of Depositor RB Owens
Passage History
Tests conducted at an early passage indicated that the cells exhibited many properties observed only in tumor lines.
Year of Origin 1973
References

Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412

Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

. Prevention and detection of cancer. vol. 2New York: Marcel Dekker; 1978.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412

Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

. Prevention and detection of cancer. vol. 2New York: Marcel Dekker; 1978.