MDOK (ATCC® CRL-1633)

Organism: Ovis aries, sheep  /  Tissue: kidney  /  Disease: normal

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Organism Ovis aries, sheep
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease normal
Gender male
Storage Conditions liquid nitrogen vapor phase
Virus Susceptibility Vesicular stomatitis New Jersey virus
Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Infectious bovine rhinotracheitis
Bluetongue virus, type 2
Complete Growth Medium Minimum essential medium (Eagle) with non-essential amino acids and sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
Name of Depositor RB Owens
References

Nelson-Rees WA, et al. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436-450, 1967. PubMed: 5587551

Madin SH, Darby NB Jr.. Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776

Nelson-Rees, W. Peculiarities of ovine cells in culture. Chromosoma 16: 79-89, 1965. PMID: 17655491

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Nelson-Rees WA, et al. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436-450, 1967. PubMed: 5587551

Madin SH, Darby NB Jr.. Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776

Nelson-Rees, W. Peculiarities of ovine cells in culture. Chromosoma 16: 79-89, 1965. PMID: 17655491