pLGdelta312-polylinker (ATCC® 87522)

Applications: YE-type (episomal) shuttle vector  /  Depositors: DJ Stillman

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Designations pLGdelta312-polylinker
Depositors DJ Stillman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 10.1999998092651400
Vector: pLGdelta312-polylinker (plasmid)
Promoters: Promoter CYC1 promoter (including CYC1 UAS)
Construction: pBR322
Marker(s):URA3,ampR
Construct size (kb): 10.19999980926514
Features: marker(s): URA3
marker(s): ampR
promoter: CYC1 promoter (including CYC1 UAS)
replicon: 2 micron ori
replicon: pMB1
coding sequence: lacZ
Applications
YE-type (episomal) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): XhoI--10.6; HindIII--8.0, 2.25; BglII--10.6.
This vector is one of three lacZ reporter plasmids that contain UAS (upstream activation site) elements (ATCC 87511, 87512 & 87522). They differ by polylinker sites.
DNA fragments can be inserted into these plasmids to screen for DNA elements that reduce promoter activity.
Growth Conditions
Temperature: 37.0°C
Related Products
component of:ATCC 87540
Shipped freeze-dried
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