MCF 10A (ATCC® CRL-10317)

Organism: Homo sapiens, human  /  Cell Type: epithelial  /  Tissue: mammary gland/breast  /  Disease: fibrocystic disease

Organism Homo sapiens, human
Tissue mammary gland/breast
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease fibrocystic disease
Age 36 years
Gender female
Ethnicity Caucasian
Applications
This line is suitable as a transfection host.
Storage Conditions liquid nitrogen vapor phase
Derivation
MCF 10A was derived from adherent cells in the population.
Clinical Data
36 years
Caucasian
female
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments
The MCF 10A cell line is a non-tumorigenic epithelial cell line.
The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.
They exhibit three dimensional growth in collagen, and form domes in confluent cultures.
Thus far, the cells have shown no signs of terminal differentiation or senescence.
The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF).
By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells.
They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies.
The calcium content of the medium exerts a strong effect on the morphology of the cells.
Complete Growth Medium The base medium for this cell line (MEBM) along with the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit. To make the complete growth medium, you will need to add the following components to the kit (sold separately):
  • 100 ng/ml cholera toxin
Note: Do not filter complete medium
Subculturing
Remove medium and rinse monolayer with PBS (ATCC 30-2200). Add 3.0 mL 0.05% trypsin, 0.53 mM EDTA and incubate at 37°C for 15 minutes. To neutralize trypsin, add 3 mL solution of 0.1% soybean trypsin inhibitor. Centrifuge cell suspension at 125 x g for 5 to 10 minutes. Resuspend cell pellet in complete culture medium. Add appropriate aliquots of cell suspension to new culture vessels.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 8,9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8,9.3
TPOX: 9,11
vWA: 15,17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1-2
PGM3, 1
Name of Depositor Michigan Cancer Foundation
Year of Origin August 22, 1984
References

Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991

Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993

Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007

Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513

Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506

Basic Documentation
References

Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991

Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993

Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007

Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513

Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506