FHs 738Lu (ATCC® HTB-157)

Organism: Homo sapiens, human  / 

Permits and Restrictions

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Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age second trimester
Gender male
Ethnicity Caucasian
Clinical Data
male
Caucasian
second trimester
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 5 to 6 days
Remove medium, add fresh 0.25% trypsin solution for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,11
D16S539: 9,11
D5S818: 11,13
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 16,18
Isoenzymes
AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
PGM1, 1
PGM3, 1
Name of Depositor RB Owens
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation