PK136 (ATCC® HB-191)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: Hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast-like
Culture Properties Suspension
Biosafety Level 1
Strain (C3H X BALB/c)F1 (B cell); BALB/c (myeloma)
Applications The antibody is specific for NK cells and is cytotoxic in the presence of complement.
Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with spleen and bone marrow cells from CE mice. 

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against mouse natural killer (NK) cells
Comments

The antibody is specific for NK cells and is cytotoxic in the presence of complement.

The pattern of reactivity of the antibody with cells of various strains of mice matches the pattern of conventional NK 1.1 antisera.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 10cells/mL. Do not allow the cell concentration to exceed 1 x 10cells/mL.
    Medium Renewal: Every 2 to 3 days
    Cryopreservation

    Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

    Culture Conditions
    Temperature: 37°C
    Isotype IgG2a
    Name of Depositor GC Koo
    References

    Koo GC, et al. The NK-1.1 (-) mouse: a model to study differentiation of murine NK cells. J. Immunol. 137: 3742-3747, 1986. PubMed: 3782794

    Koo GC, Peppard JR. Establishment of monoclonal anti-NK-1.1 antibody. Hybridoma 3: 301-303, 1984. PubMed: 6500587

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Koo GC, et al. The NK-1.1 (-) mouse: a model to study differentiation of murine NK cells. J. Immunol. 137: 3742-3747, 1986. PubMed: 3782794

    Koo GC, Peppard JR. Establishment of monoclonal anti-NK-1.1 antibody. Hybridoma 3: 301-303, 1984. PubMed: 6500587