AdEasy® System

A faster, simpler route to gene transfer and expression in mammalian cells

The AdEasy system, developed by Dr. Bert Vogelstein and associates at the Kimmel Cancer Center at Johns Hopkins, employs the recombination processes in E. coli to produce recombinant adenovirus quickly and efficiently (Proc. Natl. Acad. Sci. USA 95: 2509-2514, 1998). The gene of interest is cloned into a shuttle vector and then inserted into the adenovirus genome backbone in the E. coli cells. The recombinant plasmid is used to transfect a packaging cell line such as 293 cells, which are also available from ATCC CRL-1573.

Using the AdEasy system for gene transfer has several advantages over other methods:

  • Adenoviruses infect a broad range of cell lines, even those that are difficult to transfect with other methods.
  • Active cell division is not required for transfection.
  • High-level gene expression can be achieved.
  • The multiple plaque assays traditionally involved in identifying and selecting recombinant adenovirus are avoided, saving weeks of work.

AdEasy Basic Kit
Catalog no. JHU-23
The basic kit contains the E. coli cells and plasmids necessary to produce recombinant virus:

    AdEasierTM-1 Cells (BJ5183 strain containing pAdEasy-1)
    pShuttle in E. coli DH10B
    pShuttle-CMV in DH10B
    pAdTrack in DH10B
    pAdTrack-CMV in DH10B

Each component is ready to streak on LB agar with the appropriate antibiotics.

AdEasy Supplement Kit
Catalog no. JHU-24
The supplement kit contains components to test the efficiency of the AdEasy system for making recombinant adenoviruses:

    E. coli BJ5183 cells
    pAdEasy-1 in E. coli DH10B
    pAdEasy-2 in DH10B
    pAdEasy-1-GFP+ßgal in DH10B
    pAdEasy-2-GFP+ßgal in DH10B

BJ5183 cells are recombination-proficient bacteria which need to be made competent before use. The four plasmids are ready to streak on LB agar with the appropriate antibiotics.

For more information and FAQ visit the AdEasy Web site.

See the complete line of products from the Johns Hopkins Special Collection.

AdEasy® is a registered trademark of the Johns Hopkins University.
AdEasierTM is a trademark of the Johns Hopkins University.