|
Name
|
ATCC®
No.
|
Hosts
|
Brief
description
|
|
Charon BS(-)
|
77446
map
|
Escherichia coli
|
Vector for high-efficiency directional cloning. Clones can be converted to plasmids by recircularization after a NotI digest.
|
|
M2371
|
87557
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
A marker swap (HIS3->ADE2) vector for altering a Saccharomyces cerevisiae host by a one-step gene disruption.
|
|
M2660
|
87559
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
A marker swap (URA3->LYS2) vector for altering a Saccharomyces cerevisiae host by a one-step gene disruption.
|
|
M3499
|
87558
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
A marker swap (URA3->ADE2) vector for altering a Saccharomyces cerevisiae host by a one-step gene disruption.
|
|
p414 CYC1
|
87380
map
|
Saccharomyces cerevisiae, Escherichia coli
|
Low copy number shuttle expression vector.
|
|
p414 GALS
|
87344
map
|
Saccharomyces cerevisiae, Escherichia coli
|
Low copy number shuttle expression vector.
|
|
p416 GALL
|
87340
map
|
Saccharomyces cerevisiae, Escherichia coli
|
Low copy number shuttle expression vector.
|
|
p416 GPD
|
87360
map
|
Saccharomyces cerevisiae, Escherichia coli
|
Low copy number shuttle expression vector.
|
|
p424 GPD
|
87357
map
|
Saccharomyces cerevisiae, Escherichia coli
|
High copy number shuttle expression vector.
|
|
p425 GALL
|
87339
map
|
Saccharomyces cerevisiae, Escherichia coli
|
High copy number shuttle expression vector.
|
|
p426 GALL
|
87341
map
|
Saccharomyces cerevisiae, Escherichia coli
|
High copy number shuttle expression vector.
|
|
pACYC177
|
37031
map
sequence
|
Escherichia coli
|
Low copy number plasmid vector containing the replication system of miniplasmid p15A.
|
|
pACYC177/ET3d/yNMT
|
87052
map
sequence
|
Escherichia coli
|
Expression system compatible with ColE1 plasmids, for cotransformation and production of myristoylated proteins.
|
|
pACYC184
|
37033
map
sequence
|
Escherichia coli
|
Low copy number plasmid vector containing the replication system of miniplasmid p15A.
|
|
pAD2
|
87469
map
|
Saccharomyces cerevisiae, Escherichia coli
|
Deleter vector for constructing designer yeast strains with a non-revertable deletion of a lys2 auxotrophic marker.
|
|
pAM34
|
77185
map
sequence
|
Escherichia coli
|
Integration vector permitting positive selection for inserts.
|
|
pATH1
|
37695
map
sequence
|
Escherichia coli
|
One of a series of vectors (ATCC 37695-37703) designed to facilitate expression of an open reading frame as a trpE fusion protein.
|
|
pATH3
|
37697
map
sequence
|
Escherichia coli
|
One of a series of vectors (ATCC 37695-37703) designed to facilitate expression of an open reading frame as a trpE fusion protein.
|
|
pATH10
|
37698
map
sequence
|
Escherichia coli
|
One of a series of vectors (ATCC 37695-37703) designed to facilitate expression of an open reading frame as a trpE fusion protein.
|
|
pATH11
|
37699
map
sequence
|
Escherichia coli
|
One of a series of vectors (ATCC 37695-37703) designed to facilitate expression of an open reading frame as a trpE fusion protein.
|
|
pATH20
|
37700
map
sequence
|
Escherichia coli
|
One of a series of vectors (ATCC 37695-37703) designed to facilitate expression of an open reading frame as a trpE fusion protein.
|
|
pBAD18
|
87393
map
sequence
|
Escherichia coli
|
One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation.
|
|
pBAD18-Cm
|
87396
map
sequence
|
Escherichia coli
|
One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation.
|
|
pBAD18-Kan
|
87397
map
sequence
|
Escherichia coli
|
One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation.
|
|
pBAD18s
|
87394
map
sequence
|
Escherichia coli
|
One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation.
|
|
pBAD22
|
87398
map
sequence
|
Escherichia coli
|
One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation.
|
|
pBAD24
|
87399
map
sequence
|
Escherichia coli
|
One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation.
|
|
pBAD28
|
87400
map
sequence
|
Escherichia coli
|
One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation.
|
|
pBAD30
|
87401
map
sequence
|
Escherichia coli
|
One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation.
|
|
pBAD33
|
87402
map
sequence
|
Escherichia coli
|
One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation.
|
|
pBBR1Tp
|
87448
map
sequence
|
broad host range, Burkholderia pseudomallei, Burkholderia cepacia, Escherichia coli
|
Broad host range cloning vector allowing blue/white color detection of recominants and carrying the trimethoprim resistance gene as a selectable marker.
|
|
pBLCAT2
|
37527
map
sequence
|
Escherichia coli
|
Plasmid to simplify construction of hybrid CAT coding sequences.
|
|
pBLCAT3
|
37528
map
sequence
|
Escherichia coli
|
Developed to simplify construction of hybrid CAT genes.
|
|
pBLCAT5
|
77412
map
sequence
|
vertebrate cells, Escherichia coli
|
Reporter plasmid for analysis of enhancer sequences in vertebrate cells, using the herpes simplex virus (HSV) thymidine kinase promoter to drive expression of the CAT reporter gene.
|
|
pBLCAT6
|
77413
map
sequence
|
vertebrate cells, Escherichia coli
|
Promoter-cloning vector for analysis of promoters in vertebrate cells, using the CAT reporter gene.
|
|
pBluescript II KS(+)/LIC
|
87047
map
sequence
|
Escherichia coli
|
Ligation-independent cloning vector.
|
|
pBR322
|
37017
map
sequence
|
Escherichia coli
|
General purpose plasmid vector.
|
|
pBSL14
|
87127
map
sequence
|
Escherichia coli
|
Plasmid with an easily purified kanR cassette (flanked by direct repeats of the pUC18 multiple cloning site) for use in construction.
|
|
pBSL15
|
87128
map
sequence
|
Escherichia coli
|
Plasmid with an easily purified kanR cassette (flanked by inverted repeats of the pUC18 multiple cloning sequence) for construction. pBSL15 and pBSL86 differ in the orientation of the repeats.
|
|
pBSL86
|
87129
map
sequence
|
Escherichia coli
|
Plasmid with an easily purified kanR cassette (flanked by inverted repeats of the pUC18 multiple cloning sequence) for construction. pBSL15 and pBSL86 differ in the orientation of the repeats.
|
|
pBSL99
|
87141
map
|
Escherichia coli
|
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
|
|
pBSL119
|
87142
map
|
Escherichia coli
|
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
|
|
pBSL121
|
87143
map
|
Escherichia coli
|
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
|
|
pBSL130
|
87145
map
|
Escherichia coli
|
Plasmid containing an easily purified spcR (strR) cassette (flanked by direct repeats of the a multiple cloning sequence) for use in construction.
|
|
pBSL141
|
87146
map
|
Escherichia coli
|
Plasmid useful for vector construction. Contains a gentamicin acetyltransferase (aacC1, gtmR) cassette flanked by multiple cloning sites duplicated in tandem orientation.
|
|
pBSL142
|
87147
map
|
Escherichia coli
|
Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Gm element (aacC1, gtmR) flanked by multiple cloning sites duplicated in tandem orientation.
|
|
pBSL159
|
87148
map
|
Escherichia coli
|
Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Ap element flanked by multiple cloning sites duplicated in inverted orientation.
|
|
pBSL168
|
87149
map
|
Escherichia coli
|
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA in vitro. Contains a beta-lactamase (ampR) cassette flanked by multiple cloning sites duplicated in inverted orientation.
|
|
pBSL175
|
87150
map
|
Escherichia coli
|
Plasmid useful for vector construction. Contains an omega-Gm element (aadA, strR, spcR) flanked by multiple cloning sites duplicated in tandem orientation.
|
|
pBSL193
|
87152
map
|
Escherichia coli
|
Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Tc element flanked by multiple cloning sites duplicated in tandem orientation.
|
|
pC194
|
37034
map
sequence
|
Bacillus sp., Staphylococcus aureus
|
General purpose plasmid vector for cloning in Bacillus.
|
|
pCAT/LIC
|
87046
map
sequence
|
Escherichia coli
|
Ligation-independent promoter-cloning vector, using chloramphenicol acetyltransferase as the reporter group.
|
|
pCM182
|
87656
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
|
|
pCM183
|
87657
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
|
|
pCM184
|
87658
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
|
|
pCM185
|
87659
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
|
|
pCM188
|
87660
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
|
|
pCM189
|
87661
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
|
|
pCM190
|
87662
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
Yeast episomal expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
|
|
pCMVbeta
|
77177
map
sequence
|
vertebrate cells, Escherichia coli
|
Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining.
|
|
pCMVtkLUC+
|
87633
map
sequence
|
vertebrate cells, Escherichia coli
|
|
|
pCS19
|
77409
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
Shuttle vector for construction of genomic libraries that permits direct selection of recombinants.
|
|
pdeltablue
|
77334
map
sequence
|
Escherichia coli
|
Expression vector producing proteins with the one additional N-terminal methionine. A modified lacZ' allows blue/white screening of colonies.
|
|
pDK101
|
77406
map
sequence
|
Escherichia coli
|
A high copy number vector useful for direct cloning of double stranded amplification products.
|
|
pDO2
|
87038
map
sequence
|
Escherichia coli
|
Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing.
|
|
pDO6
|
87039
map
sequence
|
Escherichia coli
|
Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing.
|
|
pDO17
|
87040
map
sequence
|
Escherichia coli
|
Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing.
|
|
pDO19
|
87041
map
sequence
|
Escherichia coli
|
Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing.
|
|
pDO184
|
87042
map
sequence
|
Escherichia coli
|
Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing.
|
|
pDO192
|
87043
map
sequence
|
Escherichia coli
|
Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing. A series of overlapping deletion segments can be generated by cutting at the Cos site with lambda terminase, then filling in ends with a alpha phosphorothioate nucleotides, cutting with a restriction enzyme that cuts on one side of the insert, and then digesting with Exo III for various intervals of time.
|
|
pDO193
|
87044
map
sequence
|
Escherichia coli
|
Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing. A series of overlapping deletion segments can be generated by cutting at the Cos site with lambda terminase, then filling in ends with a alpha phosphorothioate nucleotides, cutting with a restriction enzyme that cuts on one side of the insert, and then digesting with Exo III for various intervals of time.
|
|
pE194
|
68359
map
sequence
|
Bacillus sp., Staphylococcus aureus
|
Natural isolate from Staphylococcus aureus that confers resistance to macrolide, lincosamide, and streptogramin type B "MLS" antibiotics.
|
|
pECV25
|
77187
map
|
vertebrate cells, Escherichia coli
|
Shuttle expression vector maintained episomally in human cells at a copy number of 25 - 50. Shows high transfection efficiency and can be introduced as supercoiled plasmids.
|
|
pEMBL8+
|
37396
map
sequence
|
Escherichia coli
|
ssDNA-producing plasmid with polylinker in lacZ'.
|
|
pEX100T
|
87436
map
sequence
|
broad host range, Escherichia coli
|
Vector to construct mutants in Pseudomonas aeruginosa.
|
|
pFL26
|
77199
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YI-type shuttle integration vector permitting visual detection of recombinants based on alpha complementation.
|
|
pFL34
|
77200
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YI-type shuttle integration vector permitting visual detection of recombinants based on alpha complementation.
|
|
pFL35
|
77201
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YI-type shuttle integration vector permitting visual detection of recombinants based on alpha complementation.
|
|
pFL36
|
77202
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
|
|
pFL38
|
77203
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
|
|
pFL39
|
77204
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
|
|
pFL44L
|
77208
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YE-type high copy number shuttle vector.
|
|
pFL44S
|
77205
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
|
|
pFL45L
|
77209
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YE-type high copy number shuttle vector.
|
|
pFL45S
|
77206
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
|
|
pFL46L
|
77210
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YE-type high copy number shuttle vector.
|
|
pFL46S
|
77207
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
|
|
pFL59+
|
77211
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA.
|
|
pFL59-
|
77212
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA.
|
|
pFL64+
|
77213
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA.
|
|
pFL64-
|
77214
map
sequence
|
Saccharomyces cerevisiae, Escherichia coli
|
YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA.
|
|
pFN476
|
86962
map
|
Escherichia coli
|
Low copy number vector to express transcripts in vivo from the T7 promoter and to minimize toxic gene effects.
|
|
pfXblue
|
77333
map
sequence
|
Escherichia coli
|
Expression vector permitting the production of a fusion protein that can be trimmed by cleavage with the coagulation factor Xa protease. A modified lacZ' allows blue/white screening of colonies.
|
|
pGEM-7Zf(+)/LIC-F
|
87048
map
sequence
|
Escherichia coli
|
Ligation-independent cloning vector.
|
|
pGEM-7Zf(+)/LIC-R
|
87049
map
sequence
|
Escherichia coli
|
Ligation-independent cloning vector.
|
|
pGEX-KN
|
77332
map
sequence
|
Escherichia coli
|
Expression vector for rapid purification of fusion proteins that contain no amino terminal extensions after thrombin cleavage. The amino acid after the initiator methionine must be charged.
|
|
pGEX-KT
|
77331
map
sequence
|
Escherichia coli
|
Expression vector for rapid purification of fusion proteins and release of proteins with fewer extraneous N-terminal amino acids.
|
|
pGFLEX
|
87629
map
sequence
|
mammalian cells, Escherichia coli
|
The pGFLEX expression system allows production of target proteins fused to either the N or C terminus of the GST pi protein and provides rapid purification of target proteins as either GST fusions or native proteins after cleavage with thrombin.
|
|
pHC79
|
37030
map
sequence
|
Escherichia coli
|
General purpose cosmid vector derived from pBR322.
|
|
pJC20
|
87113
map
sequence
|
Escherichia coli
|
High-copy-number expression vector (T7-based) that can be used for preparation of nested deletions.
|
|
pJC40
|
87114
map
sequence
|
Escherichia coli
|
High-copy-number expression vector (T7-based) that can be used for preparation of nested deletions.
|
|
pJDA
|
87575
map
sequence
|
Escherichia coli
|
One of 9 pUC19 derivatives, designed to produce PCR amplified DNA fragments that are identical except for a single base change at nt 272 (ATCC 87575 - 87578) or an insertion of 1 to 5 bases starting at nt 274 (ATCC 87579 - 87583).
|
|
pJDC
|
87576
map
sequence
|
Escherichia coli
|
One of 9 pUC19 derivatives, designed to produce PCR amplified DNA fragments that are identical except for a single base change at nt 272 (ATCC 87575 - 87578) or an insertion of 1 to 5 bases starting at nt 274 (ATCC 87579 - 87583).
|
|
pJDG
|
87577
map
sequence
|
Escherichia coli
|
One of 9 pUC19 derivatives, designed to produce PCR amplified DNA fragments that are identical except for a single base change at nt 272 (ATCC 87575 - 87578) or an insertion of 1 to 5 bases starting at nt 274 (ATCC 87579 - 87583).
|
|
pJDT
|
87578
map
sequence
|
Escherichia coli
|
One of 9 pUC19 derivatives, designed to produce PCR amplified DNA fragments that are identical except for a single base change at nt 272 (ATCC 87575 - 87578) or an insertion of 1 to 5 bases starting at nt 274 (ATCC 87579 - 87583).
|
|
pJDTiCA
|
87580
map
sequence
|
Escherichia coli
|
One of 9 pUC19 derivatives, designed to produce PCR amplified DNA fragments that are identical except for a single base change at nt 272 (ATCC 87575 - 87578) or an insertion of 1 to 5 bases starting at nt 274 (ATCC 87579 - 87583).
|
|
pJDTiCAT
|
87581
map
sequence
|
Escherichia coli
|
One of 9 pUC19 derivatives, designed to produce PCR amplified DNA fragments that are identical except for a single base change at nt 272 (ATCC 87575 - 87578) or an insertion of 1 to 5 bases starting at nt 274 (ATCC 87579 - 87583).
|
|
pJDTiCTGA
|
87582
map
sequence
|
Escherichia coli
|
One of 9 pUC19 derivatives, designed to produce PCR amplified DNA fragments that are identical except for a single base change at nt 272 (ATCC 87575 - 87578) or an insertion of 1 to 5 bases starting at nt 274 (ATCC 87579 - 87583).
|
|
pJDTiCTGCA
|
87583
map
sequence
|
Escherichia coli
|
One of 9 pUC19 derivatives, designed to produce PCR amplified DNA fragments that are identical except for a single base change at nt 272 (ATCC 87575 - 87578) or an insertion of 1 to 5 bases starting at nt 274 (ATCC 87579 - 87583).
|
|
pJDTiT
|
87579
map
sequence
|
Escherichia coli
|
One of 9 pUC19 derivatives, designed to produce PCR amplified DNA fragments that are identical except for a single base change at nt 272 (ATCC 87575 - 87578) or an insertion of 1 to 5 bases starting at nt 274 (ATCC 87579 - 87583).
|
|
pJIR418
|
77387
map
sequence
|
Clostridium perfringens, Escherichia coli
|
Shuttle expression vector permitting visual detection of recombinants in E. coli using lacZalpha complementation. Does not contain an intact beta-lactamase (bla) gene.
|
|
pJK148
|
86956
map
sequence
|
Schizosaccharomyces pombe, Escherichia coli
|
Shuttle vector for integrating sequences at leu1. Permits visual detection of recombinants by lacZalpha complementation.
|
|
pJK210
|
86957
map
sequence
|
Schizosaccharomyces pombe, Escherichia coli
|
Shuttle vector for integrating sequences at ura4. Permits visual detection of recombinants by lacZalpha complementation.
|
|
pJQ200
|
77482
map
sequence
|
broad host range, Escherichia coli
|
Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration.
|
|
pKKM13(+)
|
87403
map
sequence
|
Escherichia coli
|
Expression vector useful for mutagenesis of cloned inserts.
|
|
pKSM710
|
87156
map
sequence
|
Escherichia coli
|
Expression vector containing a 2.57 kb BglII-BspHI cassette useful for constructing a variety of expression vectors.
|
|
pKSM711
|
87157
map
sequence
|
Escherichia coli
|
Expression vector.
|
|
pKSM712
|
87158
map
sequence
|
Escherichia coli
|
Expression vector.
|
|
pKSM713
|
87159
map
sequence
|
Escherichia coli
|
Expression vector.
|
|
pKSM714
|
87160
map
sequence
|
Escherichia coli
|
Expression vector.
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pKSM715
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87161
map
sequence
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Escherichia coli
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Expression vector.
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pKSM717
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87162
map
sequence
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Escherichia coli
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Expression vector allowing generation of N-terminal deletions or modifications of foreign DNA cloned into the multiple cloning site.
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pKSM718
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87163
map
sequence
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Escherichia coli
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Expression vector allowing generation of N-terminal deletions or modifications of foreign DNA cloned into the multiple cloning site.
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pKSS
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87446
map
sequence
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Escherichia coli
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High efficiency positive selection cloning vector encoding a gene for dominant sensitivity to p-chloro-phenylalanine (No. 13,071-0, Aldrich, Milwaukee, WI).
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pLDR20
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87205
map
sequence
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Escherichia coli
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Helper plasmid with an XhoI promoter cassette containing the gene for cI857 and the lambda PR promoter.
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pLDR21
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87206
map
sequence
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Escherichia coli
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Helper plasmid with an XhoI promoter cassette containing the gene for lacIq and the ptac promoter.
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pLDR23
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87208
map
sequence
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Escherichia coli
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Helper plasmid with an XhoI promoter cassette containing the T7 phi10 promoter.
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pLDR30
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87202
map
sequence
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Escherichia coli
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Helper plasmid with a NotI cassette containing a replication origin from pBR322.
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pLDR31
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87203
map
sequence
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Escherichia coli
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Plasmid with a NotI cassette (3.451 kb) containing an R1 replication origin.
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pLEX1B
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87198
map
sequence
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Escherichia coli
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Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with 'lacZ. pLEX1B, pLEX2B, and pLEX3B (ATCC 87198-87200) differ in the reading frame established at the XmnI site.
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pLEX2B
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87199
map
sequence
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Escherichia coli
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Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with 'lacZ. pLEX1B, pLEX2B, and pLEX3B (ATCC 87198-87200) differ in the reading frame established at the XmnI site.
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pLEX3B
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87200
map
sequence
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Escherichia coli
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Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with 'lacZ. pLEX1B, pLEX2B, and pLEX3B (ATCC 87198-87200) differ in the reading frame established at the XmnI site.
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pLEX5B
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87201
map
sequence
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Escherichia coli
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Expression vector with efficient prokaryotic expression signals upstream of the XmnI site and rrnB terminators downstream.
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pLS88
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86980
map
sequence
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broad host range, Escherichia coli
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Broad host range plasmid containing three antibiotic resistance markers. Natural isolate from Haemophilus ducreyi CH37.
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pLUC+
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87630
map
sequence
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vertebrate cells, Escherichia coli
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Promoter-cloning vector for analysis of eukaryotic promoters, using the luciferase reporter gene.
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pLUC/LIC
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87045
map
sequence
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Escherichia coli
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Ligation-independent promoter-cloning vector, containing the luciferase coding sequence downstream from the cloning site.
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pMLP9
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87445
map
sequence
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Escherichia coli
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This is a postive selection cloning vector which will confer chloramphenicol resistance (recommended concentration is 10ug/ml) only when an insert has been successfully cloned into the cI repressor gene.
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pMR101
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87115
map
sequence
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Escherichia coli
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Expression vector (T7-based) with a kanR marker and a P15A replicon compatible with ColE1-derived plasmids. Particularly useful for co-transformation with ColE1-based ampR T7 expression vectors and the production of two proteins in the same cell.
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pMR102
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87116
map
sequence
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Escherichia coli
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Expression vector (T7-based) with a kanR marker and a P15A replicon compatible with ColE1-derived plasmids. Particularly useful for co-transformation with ColE1-based amp |