ATCC Clone Sets — enabling better research

As a global Biological Resource Center, ATCC has amassed an array of clone, vector and host sets that are used in a variety of research projects. ATCC has placed them in this category though they can represent diverse areas of research. A brief description of some of these sets included in this section may be found below. A complete list using descriptive names of all the sets follows. More detailed information may be found by clicking on the links.

Yeast Shuttle Expression Vectors (ATCC^® No. 87669)

Set consists of 31 yeast expression vectors that differ in promoters (GPD, TEF, ADH, CYC1), selection markers (HIS3, TRP1, LEU2, URA3) and replicons (CEN6/ARSH4, 2 micron). Distributed in E. coli.

S. cerevisae Delta0 Deleter Vector Set (ATCC^® No. 87472)

Set consists of five “deleter” vectors which are used to generate designer S. cerevisiae host strains that have non-revertible auxotrophic marker mutations for LEU2, LYS2, MET15, URA3 or ADE2. Distributed in E. coli.

AfCS pSLIK Vectors for Conditional RNA Interference (ATCC^® No. MBA-268)

Set includes 33 vectors for use in a lentviral vector platform enabling microRNA-based conditional RNA interference. (Alliance for Cellular Signaling)

AfCS Set of Subcellular Localization Markers (ATCC^® No. MBA-91)

This set of subcellular localization markers comprises 64 expression plasmids developed by the AfCS (Alliance for Cellular Signaling) as organelle markers for microscopy studies in mammalian cells .The plasmids encode either full-length or partial cDNAs tagged with a choice of fluorescent marker (either CFP or YFP). In many cases the organelle markers are provided as a set of 4 to give a choice of promoter (CMV or EF1) as well as fluorescent marker.

Microarray Control RNA Set (ATCC^® No. 87840)

A set of 11 clones of prokaryotic genes used to make RNA transcripts. Such transcripts are frequently used as controls for microarray analysis.

S. pombe Expression Vectors (ATCC^® No. 87685)

Consists of vectors that allow the fusion of a protein of interest to a triple HA epitope or a GST domain. A compatible series of restriction sites and a strategic design provides easy customization for individual applications. Use for analyzing a protein of interest or investigating gene function in yeast.

S. pombe Hosts (ATCC^® No. 47104)
This collection of S. pombe hosts allows for easy customization of experiments leading to optimal protein expression when used with a variety of different vectors systems.

S. pombe General Purpose Vectors (ATCC^® No. 87686)
This variety of pUC-based vectors, designed for maintenance in S. pombe, can be used for both gene bank construction and subcloning.