ATCC: Hosts

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Hosts

Transformation hosts, highlights

Category: Transformation host with non-revertable mutations for auxotrophic markers.

Saccharomyces cerevisiae

Features:

Complete deletion of commonly used auxotrophic marker genes (delta0) to prevent undesired gene conversion: leu2delta0, lys2delta0, met15delta0, ura3delta0.
Deletions in other common marker genes: ade2delta::hisG, his3delta200, trp1delta63.
Multiple combinations of marker gene deletions.
Either a or alpha mating type.
All strains directly descended from S288C.
Useful as transformation hosts or for construction of gene knockouts.

Availability:

The following hosts are available:

Designation	Genotype	Depositor(s)	ATCC^® No.
BY4700	MATa ura3delta0	Boeke	200866
BY4702	MATalpha leu2delta0 lys2delta0 met15delta0	Boeke	200867
BY4704	MATa ade2delta::hisG his3delta200 leu2delta0 lys2delta0 met15delta0 trp1delta63	Boeke	200868
BY4705	MATalpha ade2delta::hisG his3delta200 leu2delta0 lys2delta0 met15delta0 trp1delta63 ura3delta0	Boeke	200869
BY4706	MATa met15delta0	Boeke	200870
BY4707	MATalpha met15delta0	Boeke	200871
BY4709	MATalpha ura3delta0	Boeke	200872
BY4710	MATa trp1delta63	Boeke	200873
BY4711	MATalpha trp1delta63	Boeke	200874
BY4712	MATa leu2delta0	Boeke	200875
BY4713	MATalpha leu2delta0	Boeke	200876
BY4714	MATa his3delta200	Boeke	200877
BY4715	MATa lys2delta0	Boeke	200878
BY4716	MATalpha lys2delta0	Boeke	200879
BY4717	MATa ade2delta::hisG	Boeke	200880
BY4718	MATa met15delta0 ura3delta0	Boeke	200881
BY4719	MATa trp1delta63 ura3delta0	Boeke	200882
BY4720	MATalpha lys2delta0 trp1delta63 ura3delta0	Boeke	200883
BY4722	MATalpha leu2delta0 ura3delta0	Boeke	200884
BY4723	MATa his3delta200 ura3delta0	Boeke	200885
BY4724	MATa lys2delta0 ura3delta0	Boeke	200886
BY4725	MATa ade2delta::hisG ura3delta0	Boeke	200887
BY4726	MATalpha ade2delta::hisG ura3delta0	Boeke	200888
BY4727	MATalpha his3delta200 leu2delta0 lys2delta0 met15delta0 trp1delta63 ura3delta0	Boeke	200889
BY4728	MATa his3delta200 trp1delta63 ura3delta0	Boeke	200890
BY4729	MATalpha his3delta200 trp1delta63 ura3delta0	Boeke	200891
BY4730	MATa leu2delta0 met15delta0 ura3delta0	Boeke	200892
BY4731	MATalpha leu2delta0 met15delta0 ura3delta0	Boeke	200893
BY4732	MATalpha his3delta200 met15delta0 trp1delta63 ura3delta0	Boeke	200894
BY4733	MATa his3delta200 leu2delta0 met15delta0 trp1delta63 ura3delta0	Boeke	200895
BY4734	MATalpha his3delta200 leu2delta0 met15delta0 trp1delta63 ura3delta0	Boeke	200896
BY4735	MATalpha ade2delta::hisG his3delta200 leu2delta0 met15delta0 trp1delta63 ura3delta0	Boeke	200897
BY4736	MATa ade2delta::hisG his3delta200 met15delta0 trp1delta63 ura3delta0	Boeke	200898
BY4737	MATalpha ade2delta::hisG his3delta200 met15delta0 trp1delta63 ura3delta0	Boeke	200899
BY4738	MATalpha trp1delta63 ura3delta0	Boeke	200900
BY4739	MATalpha leu2delta0 lys2delta0 ura3delta0	Boeke	200901
BY4740	MATa leu2delta0 lys2delta0 ura3delta0	Boeke	200902