ATCC: STR Profile Database

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STR Profile Database

STR Database for Human Cell Lines

As part of our continuing efforts to fully characterize and authenticate the cell lines in our collection, ATCC is developing a comprehensive database of DNA fingerprints of STR (short tandem repeat) loci for all of our human cell lines.

STR loci are among the most informative polymorphic markers in the genome. The fingerprinting process used at ATCC involves simultaneously amplifying eight STR loci and the amelogenin gene in a multiplex PCR reaction (Promega PowerPlex® 1.2 system) which allows for discrimination of fewer than 1 in 10⁸ individuals (Lins, A.M., et al. J. Forensic Sci. 43: 1168-1180, 1998). The amplicons are separated by electrophoresis and analyzed using Genotyper® 2.0 software from Applied Biosystems. Each peak in the resulting electropherogram represents an allele that is alphanumerically scored and entered into our database.

Below is the alphanumeric data for each cell line we have characterized. This list will be updated monthly as new information is generated until all of our human cell lines are listed. As in the past, when we find an inconsistency in the fingerprint of a cell line (i.e., the fingerprint is similar or identical to that of an unrelated cell line) we will post a note at Misidentified Cell Lines. We fully expect this database will initiate and facilitate discussions on cell line authenticity.

We are providing this data to the scientific community as a service to help ensure the quality and integrity of research. You can now fingerprint your cells and compare them to our cells. We anticipate that this information will become the standard for identifying human cell lines. If you have any questions about STR loci or this database, please contact a technical service representative.



Please define your search by selecting a single choice from at least one of the below STR loci menus and/or entering an ATCC Number or Designation. Note: the more STR loci you define, the narrower your search becomes. All fields are optional.

Enter ATCC Number:

Enter Designation:

Select Amelogenin:

Select CSF1PO:

Select D13S317:

Select D16S539:

Select D5S818:

Select D7S820:

Select TH01:

Select TPOX:

Select vWA:

Disclaimer:
This database is provided for the purpose of allowing researchers to confirm the identity of ATCC cell lines being propagated in their laboratories. Reference to this database and the data contained therein may be cited in publications, and ATCC encourages such citation or reference. While every reasonable effort has been made to assure the accuracy of these data no warranty, express or implied, is made by ATCC as to their accuracy.

While ATCC has used the Promega PowerPlex Version 1.2 product in the creation of these data and recommends that researchers wishing to produce data for comparison also use this product, ATCC does not provide a general endorsement of this product or provide any warranty or representation regarding its quality or performance in the scientific community for the identification of human cell lines.

GenePrint and PowerPlex are registered trademarks of the Promega Corporation.
Genotyper is a registered trademark of Applied Biosystems.