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Animal Viruses and Antisera
ATCC® Number: VR-1616™  Price: $240.00
Classification: Parvoviridae, Dependovirus, Adeno-associated virus-2
Agent: Recombinant Adeno-associated virus 2
Strain: Recombinant Adeno-associated Virus 2 Reference Standard Stock (rAAV2 RSS)
Depositors: Adeno-associated Virus Reference Standard Working Group (AAVRSWG)
Biosafety Level: 2
Shipped: frozen Ship on Dry Ice
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Incubation : 37.0°C
Store at : -70.0°C or colder
Comments : While the final rAAV2 RSS preparation was found to be pure and free of adventitious agents, the cell harvest from which it was made tested positive for Mycoplasma arginini. The rAAV2 RSS has therefore been exposed to mycoplasma, but the purified rAAV2 RSS was found by extensive testing to be free of viable mycoplasma and mycoplasma DNA. The AAVRSWG has recommended distribution of the purified reference material because the purification process includes several steps likely to be inactivating for mycoplasma. Since the material is not intended for human use, the exposure to mycoplasma does not preclude its use for control and comparison of in vitro tests.
References : 16172546: Burger C. et al. Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous system.Molecular Therapy : 10(2):302-17, 2004 PubMed: 15294177
16172640: Chadeuf G, et al. Efficient recombinant adeno-associated virus production by a stable rep-cap HeLa cell line correlates with adenovirus-induced amplification of the integrated rep-cap genome. J. Gene Med. 2(4):260-268, 2000. PubMed: 10953917.
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All prices are listed in U.S. dollars and are subject to change without notice. A discount off the current list price will be applied to most cultures for nonprofit institutions in the United States. Cultures that are ordered as test tubes or flasks will carry an additional laboratory fee. Fees for permits, shipping, and handling may apply.
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