Product Description

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Vector
ATCC® Number: MBA-331™ Price: $190.00
Designations: pTR-UF-11 plasmid in E. coli
Biosafety Level: 1
Vector Information: Cloning sites: NotI sites (nt 1922 and 2654), AccI (nt 2862 and 3961)Transcription terminators: SV40 polyA, bovine Growth Hormone PolyASuggested Host: SURE cells from StratageneNote: Vector harbors AAV2 Inverted Terminal Repeats
Map
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: SmaI cuts in both AAV ITRs (these can be unstable in bacteria) and will serve as a diagnostic that the ITRs are intact. One or the other ITRs may be deleted (and so will the SmaI site) during culturing and cloning, and will generate other fragments (higher mol. wt.). A missing ITR can be tolerated in about 20% of your plasmid prep. When culturing the bacteria containing AAV ITRs: do not culture for more than 16-18 hours at 37 C because deletion occurs in stationary cultures
Growth Conditions: ATCC medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Temperature: 37°C
Related Products: ATCC VR-1616
ATCC CRL-2972
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All prices are listed in U.S. dollars and are subject to change without notice. A discount off the current list price will be applied to most cultures for nonprofit institutions in the United States. Cultures that are ordered as test tubes or flasks will carry an additional laboratory fee. Fees for permits, shipping, and handling may apply.
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