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Cell Biology
ATCC® Number: HTB-180™    Price: $338.00
Designations: NCI-H345 [H345]
Depositors:  AF Gazdar, JD Minna
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, multicell aggregates and some adherent cells (This cell line grows as gland-like clusters in suspension with a few attached cells. The viability of the cells in clusters is better than the viability of the single cells in the cultures.)
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: lung
Disease: carcinoma; small cell lung cancer
Derived from metastatic site: bone marrow
Cellular Products: gastrin releasing peptide
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1981
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 8
D5S818: 11,12
D7S820: 9,13
THO1: 9,9.3
TPOX: 8,9
vWA: 15,16
Cytogenetic Analysis: hypotriploid; modal number = 63; range = 60 to 66.
Sixteen or more marker chromosomes were common to most cells. Nearly half of the markers had lengths close to the D group or smaller chromosomes. Among the identifiable markers were der(1)t(1;17)(q21;q11), der(?9)t(1;?9)()q12;?q21, t(8q?12q), t(12q?17q), der(9)t(9,?)(q22;?). Normal N13 was absent; the N8, N9, N12 and N19 were found as single copies; N20 generally had 4 copies; there were 2 normal X chromosomes and no Y chromosome was detected.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1-2
PGM3, 1
Age: 64 years adult
Gender: male
Ethnicity: Caucasian
Comments: The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue.
The line does not exhibit any gross structural DNA abnormalities.
The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
Propagation: ATCC complete growth medium: HITES medium (serum free) HITES medium is formulated at the ATCC as follows: Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006) Insulin 0.005 mg/ml Transferrin 0.01 mg/ml Sodium selenite 30 nM Hydrocortisone 10 nM beta-estradiol 10 nM HEPES 10 mM L-glutamine 2 mM (in addition to that in the base medium)
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium. Aggregates may be dispersed by trituration. The use of trypsin is not recommended.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257
23162: Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893
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