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Cell Biology
ATCC® Number: CRL-5973™    Price: $417.00
Designations: SNU-5
Depositors:  J Park
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, multicell aggregates
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: stomach
Disease: gastric carcinoma
Derived from metastatic site: ascites
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Isolation: Isolation date: 1987
Receptors: acetylcholine, muscarinic, expressed
vasoactive intestinal peptide (VIP), expressed
vasoactive intestinal peptide (VIP); acetylcholine, muscarinic
Oncogene: myc +; erb B2 +
Antigen Expression: Blood Type O; Rh +
The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 8,9
D16S539: 13
D5S818: 10
D7S820: 8,12
THO1: 9
TPOX: 11
vWA: 15,16
Cytogenetic Analysis: This is a hypotetraploid human cell line with the modal chromosome number of 87 and 88 occurring in a total of 36% of cells. Cells having higher ploidies were found at 9.6%. Eighteen or more marker chromosomes were found in most cells, including paired der(1)t(1;21) (p36.3;q11.2); der(1)t(1;?) (p32;?); 7q+'s; del(6) (q23); 12q+; and i(17q); triple der(2)t(2;?13) (p25.3;q14.3); and other single markers. There are two kinds of 7q+'s: der(7)t(7;?) (q22.3;?) and der (7)t(7;?) (q32;?). Multiple copies of DMs were also found in most cells. Normal N1 was absent. N5, N7, N10, N19 and N20 had four copies in most cells. At least one normal X chromosome was detected, but the normal Y was absent.
Age: 33 years
Gender: female
Ethnicity: Asian
Comments: SNU-5 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The patient had previously received chemotherapy including 5-fluorouracil, doxorubicin and mitomycin-C. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-5 cells were positive for VIP receptors but lacked gastrin receptors. They expressed receptors for muscarinic cholinergic agents. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The cells expressed levels of c-myc and c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 34 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397
23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..
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