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Cell Biology
ATCC® Number: CRL-2876™    Price: $328.00
Designations: VCaP
Depositors:  KJ Pienta
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: prostate
Tissue: vertebral metastasis
Disease: cancer
Cell Type: epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1997
Tumorigenic: Yes
Antigen Expression: cytokeratin-18; Homo sapiens, expressed
p53 antigen; Homo sapiens, expressed
prostate specific antigen (PSA); Homo sapiens, expressed
prostatic acid phosphatase (PAP); Homo sapiens, expressed
Rb protein; Homo sapiens, expressed
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19
Age: 59 years
Gender: male
Ethnicity: Caucasian
Comments: This line was established in 1997 from a vertebral bone metastasis from a patient with hormone refractory prostate cancer. It was passaged as xenografts in mice then cultured in vitro. It is androgen sensitive in vitro and in vivo.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Hank's Balanced Salt Solution or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    An inoculum of 2 X 10(4) to 4 X 10(4) viable cells/sq. cm is recommended.
  6. Incubate cultures at 37°C.

Interval: Subculture when the cell concentration reaches between 1 X 10(5) and 2 X 10(5) cells/sq. cm.
Subcultivation Ratio: 1:3 to 1:4
Preservation: Freeze medium: 95% growth medium; 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: about 53 hours
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
Cell culture tested DMSO:ATCC 4-X
Erythrosin B vital stain solution:ATCC 30-2404
Trypan Blue vital stain solution:ATCC 30-2402
References: 92253: Korenchuk S, et al. VCaP, a cell-based model system of human prostate cancer. In Vivo 15: 163-168, 2001. PubMed: 11317522
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