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Cell Biology
ATCC® Number: CRL-2434™    Price: $399.00
Designations: 1G3
Depositors:  PJ Bjorkman
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed: adherent and suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against FcRn heavy chain heterodimers
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Comments: The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992.
Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn.
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).
The antibody recognizes FcRn heavy chain heterodimers.
FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules.
The IG3 (CRL-2434) antibody and the 4C11 (CRL-2436) antibody recognize two lipid linked forms of FcRn.
These forms are truncated FcRn heavy chain paired with beta 2-microgloublin-DAF (FcRn beta-2 microglobulin DAF) and lipid-linked FcRn paired with unaltered beta 2-microgloublin (FcRn-DAF/beta 2-microgloublin).
They also recognize the FcRn-DAF construct.
The 2G3 (CRL-2435) antibody fails to recognize the FcRn heterodimer form in which the heavy chain is attached to the lipid anchor (FcRn-DAF/beta 2-microgloublin) and it also fails to recognize the FcRn-DAF construct.
Unlike 4C9 (CRL-2437), the monoclonal antibodies IG3 (CRL-2434), 2G3 (CRL-2435) and 4C11 (CRL-2436) do not recognize rat beta 2-microglobulin alone or complexed with FcRn heavy chains.
This antibody is used in studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG. [39787]
Propagation: ATCC complete growth medium: HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from BioWhittaker, Walkersville, MD 21793, 800-638-7184.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 every 3 - 4 day is recommended
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 X 10exp5 viable cells/ml.
Preservation: HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%.
Related Products: recommended serum:ATCC 30-2020
References: 39787: Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418
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