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Cell Biology
ATCC® Number: CRL-2294™    Price: $417.00
Designations: BCP-1
Depositors:  SJ Gao, PS Moore, Y Chang
Biosafety Level: 2 [CELLS CONTAIN HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: body cavity based lymphoma
Cell Type: B lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1995
Age: 92 years
Gender: male
Comments: BCP-1 is a clonal lymphoma cell line isolated in 1995 by serial dilution of peripheral blood mononuclear cells from an HIV seronegative patient with body cavity based lymphoma (BCBL).
The BCP-1 cell line is positive for the Kaposi's sarcoma associated herpesvirus (KSHV, provisionally designated Human Herpes Virus-8 or HHV-8) genome at 150 copies/cell.
BCP-1 is negative for Epstein-Barr virus (EBV), HHV-6 and HHV-7.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml.
Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 24391: Gao SJ, et al. KSHV antibodies among Americans, Italians and Ugandans with and without Kaposi's sarcoma. Nat. Med. 2: 925-928, 1996. PubMed: 8705864
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