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Cell Biology
ATCC® Number: CRL-2277™    Price: $417.00
Designations: BC-3
Depositors:  Cornell Res. Fndn., Inc.
Biosafety Level: 2 [Cells contain Kaposi's sarcoma-associated herpesvirus genome ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Disease: lymphoma
Cell Type: B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Antigen Expression: CD30 +; CD38 +; CD45 +; CD 54 +; CD71 +; HLA-DR +; EMA + (epithelial membrane antigen); CD2 -; CD3 -; CD4 -; CD5 -, CD8 -; CD19 -; CD20 -; CD21 -; CD22 -
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 14,18
Age: 85 years
Gender: male
Ethnicity: Caucasian
Comments: BC-3 is a primary effusion lymphoma cell line originated in 1995 by Leandros Arvanitakis and Ethel Cesarman.
The cells were established from pleural effusion from a human immunodeficiency virus (HIV) negative patient diagnosed with primary effusion (body cavity based) lymphoma (PEL).
The BC-3 cell line contains the viral genome for Kaposi's sarcoma-associated herpesvirus (KSHV, provisionally designated HHV-8).
KSHV is a recently identified and partially uncharacterized virus.
BC-3 cells are negative for both HIV and Epstein-Barr virus (EBV).
They are also reported to be negative for herpes simplex virus 1 & 2 (HSV-1 and HSV-2) and cytomegalovirus (CMV).
The BC-3 cell line allows in vitro culture of KSHV viral genomes in the absence of EBV.
It is ideal for the isolation and study of KSHV.
BC-3 has an intact KSHV genome of about 170 kb both in the cell line and in the viral isolate.
The cells do not express B-cell lineage restricted antigens or kappa or lambda immunoglobulin light chains) or T-cell lineage-restricted antigens.
The cells do express activation antigens.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23359: Arvanitakis L, et al. Establishment and characterization of a primary effusion (body cavity- based) lymphoma cell line (BC-3) harboring kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) in the absence of Epstein-Barr virus. Blood 88: 2648-2654, 1996. PubMed: 8839859
39036: Cesarman E, et al. KSHV positive cell lines. US Patent 5,908,773 dated Jun 1 1999
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