Product Description

Before submitting an order you will be asked to read and accept the terms and conditions of ATCC's Material Transfer Agreement or, in certain cases, an MTA specified by the depositing institution.
Customers in Europe, Australia, Canada, China, Hong Kong, India, Israel, Japan, Korea, Macau, Mexico, New Zealand, Singapore, and Taiwan, R.O.C. must contact a local distributor for pricing information and to place an order for ATCC cultures and products.



Cell Biology
ATCC® Number: CRL-2108™    Price: $458.00
Designations: EHS
Depositors:  H Kleinman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Organism: Mus musculus (mouse)
Morphology: fibroblast
Source: Tissue: connective tissue
Strain: C57BL
Disease: sarcoma
Cellular Products: laminin; collagen IV; entactin; heparin sulfate proteoglycan
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: EHS (Englebreth-Holm-Swarm) is a sarcoma carried as a transplantable tumor in C57BL mice.
It produces all the basement membrane components (laminin, collagen IV, entactin, and heparan sulfate proteoglycan).
The EHS sarcoma is a source of Matrigel, an extract that stimulates cell differentiation.
Matrigel is rich in laminin, growth factors, entactin/nidogen, type IV collagen, and heparan sulfate proteoglycan.
The EHS sarcoma is a rich source of both individual basement membrane components and Matrigel.
The ATCCstocks were established from tumors grown in C57BL/6J mice.
Propagation: ATCC complete growth medium: Duration: These cell are propagated in C57BL/6 mice.
Subculturing: Protocol: Harvest tumor from one mouse, mince and inject intramuscularly (over the hindlimb) into 3 to 10 C57BL/6 mice. Examine the mice daily for tumor progression, and harvest tumors (usually about 14 to 21 days after inoculation).
Preservation: Freeze medium: Ham's F12K medium, 80%; fetal bovine serum; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
References: 22608: Kleinman HK, et al. Basement membrane complexes with biological activity. Biochemistry 25: 312-318, 1986. PubMed: 2937447
22966: . . J. Tissue Culture Methods 16: 227-230, 1994.
23396: Timpl R, et al. Laminin--a glycoprotein from basement membranes. J. Biol. Chem. 254: 9933-9937, 1979. PubMed: 114518
Return to Top


Notices and Disclaimers

ATCC products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this site, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate.

All prices are listed in U.S. dollars and are subject to change without notice. A discount off the current list price will be applied to most cultures for nonprofit institutions in the United States. Cultures that are ordered as test tubes or flasks will carry an additional laboratory fee. Fees for permits, shipping, and handling may apply.
Back to my Search