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Cell Biology
ATCC® Number: CRL-2026™    Price: $349.00
Designations: Hepa-1c1c7
Depositors:  O Hankinson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: epithelial
Source: Strain: C57L
Organ: liver
Disease: hepatoma
Cellular Products: cytochrome P450IA1
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Receptors: aryl hydrocarbon (Ah)
Comments: This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.
The cells are highly inducible for cytochrome P450IA1.
This and mutant derivatives of it are useful for studying the aryl hydrocarbon (Ah) receptor and P450IA1 regulation.
Propagation: ATCC complete growth medium: Alpha minimum essential medium without nucleosides, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 18 hrs
Related Products: recommended serum:ATCC 30-2020
References: 22320: Bernhard HP, et al. Expression of liver phenotypes in cultured mouse hepatoma cells: synthesis and secretion of serum albumin. Dev. Biol. 35: 83-96, 1973. PubMed: 4362668
22342: Hankinson O, et al. Genetic and molecular analysis of the Ah receptor and of Cyp1a1 gene expression. Biochimie 73: 61-66, 1991. PubMed: 1851644
22593: Hankinson O. Single-step selection of clones of a mouse hepatoma line deficient in aryl hydrocarbon hydroxylase. Proc. Natl. Acad. Sci. USA 76: 373-376, 1979. PubMed: 106390
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All prices are listed in U.S. dollars and are subject to change without notice. A discount off the current list price will be applied to most cultures for nonprofit institutions in the United States. Cultures that are ordered as test tubes or flasks will carry an additional laboratory fee. Fees for permits, shipping, and handling may apply.
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