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Cell Biology
ATCC® Number: CRL-1932™    Price: $273.00
Designations: 786-O [786-0]
Depositors:  RD Williams
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: kidney
Disease: renal cell adenocarcinoma
Cellular Products: parathyroid hormone (PTH) like peptide
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (technology from amaxa)
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 8
D16S539: 12
D5S818: 9
D7S820: 11,12
THO1: 6,9.3
TPOX: 8,11
vWA: 15,17
Cytogenetic Analysis: hypertriploid; Y was present in 60% the cells examined
Age: 58 years
Gender: male
Ethnicity: Caucasian
Comments: This line was derived from a primary clear cell adenocarcinoma.
The cells display both microvilli and desmosomes, and can be grown in soft agar.
The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors.
The peptide has an N terminal sequence similar to PTH, has PTH like activity, and has a molecular weight of 6000 daltons.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

      Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
      Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22648: Williams RD, et al. In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed: 1010528
22653: Williams RD, et al. In vitro cultivation of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102
23304: Thiede MA, et al. Human renal carcinoma expresses two messages encoding a parathyroid hormone-like peptide: evidence for the alternative splicing of a single- copy gene. Proc. Natl. Acad. Sci. USA 85: 4605-4609, 1988. PubMed: 3290897
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