Search Catalog
Product Description

Before submitting an order you will be asked to read and accept the terms and conditions of ATCC's Material Transfer Agreement or, in certain cases, an MTA specified by the depositing institution.
Customers in Europe, Australia, Canada, China, Hong Kong, India, Israel, Japan, Korea, Macau, Mexico, New Zealand, Singapore, and Taiwan, R.O.C. must contact a local distributor for pricing information and to place an order for ATCC cultures and products.



Cell Biology
ATCC® Number: CRL-1520™    Price: $323.00
Designations: A2B5 clone 105
Depositors:  GS Eisenbarth, M Nirenberg, FS Walsh
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
Morphology: lymphoblast
Source: Cell Type: hybridoma:lymphoblast B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a glycolipid antigen on neuronal cell bodies (chick, rat, bovine and human), pancreatic islet cells (rat, human, bovine), thymic epithelium (rat, human)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Antigen Expression: H-2d
Comments: Animals were immunized with 8 day chick embryo retina cells.
Spleen cells were fused with P3X63Ag8 myeloma cells.
In the rat, A2B5 reacts with gangliosides expressed in brain, pituitary, pancreatic islets, thymic endocrine epithelium, kidney glomeruli and the adrenal medulla.
Increasing expression of the A2B5 reactive ganglioside correlates with increasing degree of differentiation of rat insulinoma cells (RIN5F).
Tested and found negative for ectromelia virus (mousepox).
This line was formerly designated ATCC HB-29.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain the cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 918: Eisenbarth GS, et al. Monoclonal antibody to a plasma membrane antigen of neurons. Proc. Natl. Acad. Sci. USA 76: 4913-4917, 1979. PubMed: 388422
1624: Powers AC, et al. Characterization of monoclonal antibody 3G5 and utilization of this antibody to immobilize pancreatic islet cell gangliosides in a solid phase radioassay. Endocrinology 114: 1338-1343, 1984. PubMed: 6368201
1638: Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562
1639: McFarland EJ, et al. The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. J. Immunol. 133: 1241-1249, 1984. PubMed: 6205074
22221: Haynes BF, et al. Identification of human and rodent thymic epithelium using tetanus toxin and monoclonal antibody A2B5. J. Clin. Invest. 71: 9-14, 1983. PubMed: 6336763
23132: McBurney MW, et al. Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. J. Neurosci. 8: 1063-1073, 1988. PubMed: 2894413
23144: Mokhtar N, et al. Thymoma: lymphoid and epithelial components mirror the phenotype of normal thymus. Hum. Pathol. 15: 378-384, 1984. PubMed: 6609116
23280: Eisenbarth GS, et al. Expression of receptors for tetanus toxin and monoclonal antibody A2B5 by pancreatic islet cells. Proc. Natl. Acad. Sci. USA 79: 5066-5070, 1982. PubMed: 6750614
23447: Haynes BF, et al. Demonstration of abnormalities in expression of thymic epithelial surface antigens in severe cellular immunodeficiency diseases. J. Immunol. 130: 1182-1188, 1983. PubMed: 6600476
23512: Bartholomeusz RK, et al. Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells. Endocrinology 124: 2680-2685, 1989. PubMed: 2541995
Return to Top


Notices and Disclaimers

ATCC products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this site, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate.

All prices are listed in U.S. dollars and are subject to change without notice. A discount off the current list price will be applied to most cultures for nonprofit institutions in the United States. Cultures that are ordered as test tubes or flasks will carry an additional laboratory fee. Fees for permits, shipping, and handling may apply.
Back to my Search