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OV-90
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University of Montreal |
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Biosafety Level: |
1
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frozen
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Medium & Serum:
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See Propagation
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adherent |
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Homo sapiens (human)
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epithelial
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Organ: ovary Tumor Stage: grade 3, stage IIIC Disease: malignant papillary serous adenocarcinoma Derived from metastatic site: ascites |
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keratin [49408]
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation date: August, 1992 |
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Yes
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her2/neu +, p53 (mutated, Ser --> Arg mutation at exon 6, codon 215) |
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Amelogenin: X CSF1PO: 12,13 D13S317: 11,12 D16S539: 11 D5S818: 11,15 D7S820: 10,10.1 THO1: 9.3 TPOX: 8,10 vWA: 16,17 |
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46, XX, der(1)t(1;10)(p36;p15), hsr(3)(p11), der(9;17)(q10;q10), der(10)t(10;17)(p15;p12p13), der(13)t(13;13)(p11;q14) [49408] |
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64 years |
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female |
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ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15% Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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Protocol: - Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 3 to 4 days
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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recommended serum:ATCC 30-2020 |
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42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998 49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993 49722: Lounis H, et al. Mapping of chromosome 3p deletions in human epithelial ovarian tumors. Oncogene 17: 2359-2365, 1998. PubMed: 9811467 |
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