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Cell Biology
ATCC® Number: CRL-11351™    Price: $407.00
Designations: H69AR
Depositors:  S Cole
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: lung
Disease: carcinoma; small cell lung cancer; multidrug resistant
Cell Type: epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
Age: 55 years adult
Gender: male
Ethnicity: Caucasian
Comments: The cell line is cross-resistant to anthracycline analogues including daunomycin, epirubicin, menogaril, and mitoxantrone as well as to acivicin, etoposide, gramicidin D, colchicine, and the Vinca alkaloids, vincristine and vinblastine. [49663]
The cells display little or no cross-resistance to bleomycin, 5-fluorouracil, and carboplatin. They have a slight collateral sensitivity to 1-dehydrotestosterone and lidocaine. [49663]
The H69AR cell line is approximately 50-fold resistant to adriamycin as compared to the parental NCI-H69 cell line. [49655]
The monoclonal antibodies QCRL-1 (ATCCHB-11765) and QCRL-3 (ATCCHB-11766) will react with fixed H69AR cells. [49661]
The antibodies reacts with MRP (multidrug resistance protein), a 190,000 integral membrane phosphoglycoprotein that is overexpressed in some drug-selected resistant cell lines [49661]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 3 to 5 times per week as culture becomes acidic
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phaseliquid nitrogen vapor phase
Doubling Time: 24 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC HTB-119
References: 49655: Deeley RG, Cole SP. Antibodies to a multidrug resistance protein. US Patent 6,063,621 dated May 16 2000
49661: Hipfner DR, et al. Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies. Cancer Res. 54: 5788-5792, 1994. PubMed: 7954400
49663: Mirski SE, et al. Multidrug resistance in a human small cell lung cancer cell line selected in adriamycin. Cancer Res. 47: 2594-2598, 1987. PubMed: 2436751
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