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Cell Biology
ATCC® Number: CRL-10685™    Price: $438.00
Designations: PG13/LN c8
Depositors:  Fred Hutchinson Cancer Res. Cntr.
Biosafety Level: 2 [CELLS CONTAIN RETROVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: fibroblast
Source: Disease: normal
Strain: NIH/Swiss
Cellular Products: a Gibbon ape Leukemia Virus (GaLV) based neomycin resistance transducing vector
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Reverse Transcript: positive
Age: embryo
Comments: PG13/LN c8 cells produce a retroviral vector (LN) that transduces the neo gene and can infect cells from many mammalian species (other than mice).
The line was derived from PG13 (see ATCCCRL-10686).
Although virus production has not been observed, there is a possibility that the cells will produce a virus similar to GaLV (a moderate risk oncogenic virus) and Biosafety Level 2 or higher precautions should be taken when using this cell line.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% Trypsin, 0.02% EDTA solution and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks.
References: 1818: Miller AD, et al. Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus. J. Virol. 65: 2220-2224, 1991. PubMed: 1850008
4473: Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression. BioTechniques 7: 980-990, 1989. PubMed: 2631796
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