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Cell Biology
ATCC® Number: CCL-86™    Price: $272.00
Designations: Raji
Depositors:  W Henle
Biosafety Level: 2 [CELLS CONTAIN HERPESVIRUS (EBV) ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1963
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Virus Resistance: The cells are partially resistant to poliovirus and vesicular stomatitis viruses.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16,19
Cytogenetic Analysis: Karyotype 100% stable within male diploid stemline of 46. Cells with 47 chromosomes frequently contained an extra "E" group chromosome.; There is 6% polyploidy and occasional disparity in the size of the homologs of the number 1 chromosome and the number 4 chromosome.
Age: 11 years
Gender: male
Ethnicity: Black
Comments: The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitt's lymphoma of the left maxilla of an 11-year-old Black male. [22142]
EBNA positive.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation. Cultures can then be established by resuspending the cells in fresh medium at 4 X 10(5) viable cells/ml. A maximum of 3 X 10(6) viable cells/ml is obtainable.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CCL-214
References: 22142: Pulvertaft JV. Cytology of Burkitt's tumour (African lymphoma). Lancet 1: 238-240, 1964. PubMed: 14086209
22169: Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790
22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523
22572: Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568
26253: Epstein MA, et al. Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). J. Natl. Cancer Inst. 37: 547-559, 1966. PubMed: 4288580
26254: . . Trans. N.Y. Acad. Sci. 29: 61, 1966.
32395: Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679
32448: Rich SA, et al. Purification, microresequencing, and immunolocalization of p36, a new interferon-alpha-induced protein that is associated with human lupus inclusions. J. Biol. Chem. 271: 1118-1126, 1996. PubMed: 8557639
16173680: U.S. Pharmacopeia USP Monographs: Technetium 99mTc Fanolesomab Injection. Rockville, MD: USP32-NF27, 2005
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