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Cell Biology
ATCC® Number: CCL-46.1™    Price: $407.00
Designations: NCTC 3749
Depositors:  VJ Evans
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: fibroblast
Source: Disease: lymphoma
Strain: DBA/2
Cellular Products: produce interleukin-1 (IL-1, interleukin 1)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance: poliovirus 1
Antigen Expression: H-2d
Comments: This line is a derivative of P388D1 (ATCCCCL-46).
When the cells are grown in chemically defined medium (RPMI 1640 medium without serum), they produce IL-1 in response to stimulation with phorbol myristic acid (PMA) and lipopolysaccharide (LPS). [869]
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: NCTC 135 medium, 100%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping. Remove one-half of the old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: NBL-maintained derivative:ATCC CRL-6441
References: 869: Mizel SB, et al. Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line P388D1. I. Enhancement of LAF production by activated T lymphocytes. J. Immunol. 120: 1497-1503, 1978. PubMed: 149171
22195: Evans VJ, et al. A new tissue culture isolation and explanation of the P388 lymphocytic neoplasm in a chemically characterized medium. Exp. Cell Res. 32: 212-217, 1963. PubMed: 14094040
22196: Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231
22208: . . Am. J. Pathol. 33: 603, 1957.
26204: Rabson AS, Leggallais FY. Cytopathogenic effect produced by polyoma virus in cultures of milk-adapted murine lymphoma cells (strain P388 D1). Proc. Soc. Exp. Biol. Med. 100: 229-233, 1959. PubMed: 13634094
26205: . . Nature 181: 1343, 1958.
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