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Cell Biology
ATCC® Number: CCL-219™    Price: $339.00
Designations: L1210
Depositors:  EM Jensen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast
Source: Disease: lymphocytic leukemia
Strain: DBA subline 212
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Cytogenetic Analysis: modal number = 40; range = 32 to 80.
Mouse karyotype with a diploid stemline number of 40 chromosomes.
Age: 8 months
Gender: female
Comments: Tested and found negative for ectromelia virus (mousepox).
The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether. [22513]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 viable cells/ml.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 4 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 8 to 10 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
References: 22513: . . J. Natl. Cancer Inst. 10: 179-192, 1949.
26055: . . J. Natl. Cancer Inst. 36: 405-421, 1966.
26056: . . Cancer Chemother. Rep. 51: 451-453, 1967.
32923: Young SW, et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865
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All prices are listed in U.S. dollars and are subject to change without notice. A discount off the current list price will be applied to most cultures for nonprofit institutions in the United States. Cultures that are ordered as test tubes or flasks will carry an additional laboratory fee. Fees for permits, shipping, and handling may apply.
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