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Vector
ATCC® Number: 87331™ Price: $190.00
Designations: p425 GAL1
Depositors: M Funk
Biosafety Level: 1
Vector Information: Size (kb): 7.5399999618530270
Vector: p425 GAL1 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pRS425, GAL1 promoter
Marker(s):LEU2,ampR
Construct size (kb): 7.539999961853027
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GAL1
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1
Map
Shipped: freeze-dried
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: YE-type (episomal) shuttle vector [4249]
YX-type (expression) shuttle vector [4249]
Comments: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9 (doublet); EcoRI--5.1, 2.4; KpnI--5.8, 1.7. It appears that the SacI site at the start of the GAL1 promoter has been lost.
Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. [4249]
High copy number shuttle expression vector. [4249]
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. [4249]
Growth Conditions: ATCC medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Temperature: 37.0°C
Related Products: component of:ATCC 87670
References: 4249: Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736
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