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Vector
ATCC® Number: 87035™ Price: $190.00
Designations: pBG1
Depositors: KL Gould
Other IDs: Nucleotide (GenBank) : L19524
Biosafety Level: 1
Vector Information: Schizosaccharomyces pombe Lindner, teleomorph
Size (kb): 5.6999998092651370
Vector: pBG1 (plasmid)
Promoters: Promoter lac
Construction: pUC18, ars1, his3
Marker(s):ampR,his3
Construct size (kb): 5.699999809265137
Features: insert detection: lacZ'
marker(s): ampR
marker(s): his3+
promoter: lac
replicon: ars1
replicon: pMB1
MCS: SacI...SphI
Shipped: freeze-dried
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: contains sequence autonomous replication sequence
contains sequence imidazole acetol phosphate transaminase
shuttle vector
Comments: Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.2, 0.55; DraI/EcoRI--2.5, 2.4, 0.7; HindIII--3.5, 2.2; PstI--5.7.
The his3 insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.49; ClaI--0.04; EcoRI--0.47, 1.01; HindIII--1.03; PvuI--0.04; XbaI--0.27; XhoI--0.84. [41755]
Growth Conditions: ATCC medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Temperature: 37.0°C
Related Products: component of:ATCC 87686
References: 41755: Burke JD, Gould KL. Molecular cloning and characterization of the Schizosaccharomyces pombe his3 gene for use as a selectable marker. Mol. Gen. Genet. 242: 169-176, 1994. PubMed: 8159167
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