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Clone
ATCC® Number: 63013  Price: $190.00
Designation: pMT70
Depositors: R Nusse
Insert Source: Mus musculus
Insert Information: DNA: cDNA
Insert lengths(kb): 2.400000095367432
Gene product: wingless-type MMTV integration site 1, human homolog [Int1]
Biosafety Level: 1
Shipped: freeze-dried plasmid in E. coli
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Classification: Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Vector: Size (kb): 3.0000000000000000
Vector: pSP6 (plasmid)
Promoters: Promoter unknown promoter
Marker(s):ampR
Features: marker(s): ampR
promoter: unknown promoter
Comments: Insert contains internal BamHI (1) and ClaI (1) sites. [3280]
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 2.4; EcoRI--5.4; HindIII--5.4; BamHI--5.4; consistent with deposited DNA.
A new nomenclature system has been proposed for Int-1 and related genes. It has been suggested that Int-1 become Wnt-1. [22802]
Contains cDNA of murine int-1 that encodes the complete protein. RNA can be synthesized in vitro from the SP6 vector promoter. [3280]
Media Description: ATCC medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
References: 2482: van Ooyen A, Nusse R. Structure and nucleotide sequence of the putative mammary oncogene int-1; proviral insertions leave the protein-encoding domain intact. Cell 39: 233-240, 1984. PubMed: 6091914
3280: Rijsewijk FA, et al. Construction of a retroviral cDNA version of the int-1 mammary oncogene and its expression in vitro. Nucleic Acids Res. 14: 693-702, 1986. PubMed: 3003692
22802: Nusse R, et al. A new nomenclature for int-1 and related genes: the Wnt gene family. Cell 64: 231, 1991. PubMed: 1846319
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